c-MYC antibody

Catalog No. ABIN920716

This Mouse Monoclonal antibody specifically detects c-MYC in WB, IHC, IF, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for c-MYC antibody (ABIN920716)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: 9E10

Product Details anti-c-MYC Antibody

Specificity: Clone 9E10 anti-c-myc specifically recognizes the 10-amino acid epitope of human c-myc amino acids 410-419.

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 1-5 µg/mL. Western blot: Use at 1-5 µg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µg of purified monoclonal antibody at 1 mg/mL in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, and 3 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: c-myc

Background: Clone 9E10 can be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells. Balb/C mice were immunized with the synthetic peptide EQKLISEEDL coupled to carrier. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by western blot. The native c-myc protein is very labile. Lysis buffers should be supplemented with protease inhibitors if detection of intact c-myc is the objective.

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect