Integrin beta 2 antibody (ITGB2)

Details for Product anti-ITGB2 Antibody No. ABIN94010, Supplier: Log in to see
Antigen
  • ITGB2
  • 2E6
  • AI528527
  • Cd18
  • LAD
  • LCAMB
  • Lfa1
  • MF17
  • CD18
  • LFA-1
  • MAC-1
  • MFI7
  • AI256028
  • LTbetaR
  • Ltar
  • TNF-R-III
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFRrp
  • Tnfbr
  • Tnfrsf3
  • D12S370
  • LT-BETA-R
  • TNFR3
  • TNFRSF3
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • integrin, beta 2
  • integrin beta 2
  • lymphotoxin B receptor
  • lymphotoxin beta receptor (TNFR superfamily, member 3)
  • ITGB2
  • Itgb2
  • CD18
  • Ltbr
  • LTBR
Alternatives
anti-Human Integrin beta 2 antibody for Blocking Reagent
Reactivity
Human
476
213
118
43
26
21
21
5
4
3
3
3
3
3
2
1
1
1
Host
Mouse
350
165
161
10
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Integrin beta 2 antibody is un-conjugated
88
72
52
20
14
11
9
7
7
7
7
6
6
6
5
5
5
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
471
285
201
146
95
89
74
68
53
40
26
17
11
9
7
6
3
2
1
1
1
1
1
1
Options
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Immunogen Peripheral blood mononuclear cells
Clone MEM-148
Isotype IgG1
Specificity The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
HLDA VI, WS Code AS A052
No Cross-Reactivity Pig (Porcine)
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name ITGB2 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Research Area Surface Receptors of Immune Cells, Hematopoietic Progenitors, CD Antigens, Stem Cells
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN94010) Western Blotting analysis of PMA-activated neutrophils (Fig. A) and monocytes (Fig. B...
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN94010) Western Blotting analysis of PMA-activated neutrophils (Fig. A) and monocytes (Fig. B...
Product cited in: Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Drbal, Angelisová, Cerný, Hilgert, Horejsí: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, 2001 (PubMed).

Drbal, Angelisová, Cerný, Pavlistová, Cebecauer, Novák, Horejsí: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, 2000 (PubMed).

Background publications Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007 (PubMed).

Peters, Sindrilaru, Wang, Oreshkova, Renkl, Kess, Scharffetter-Kochanek: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006 (PubMed).

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005 (PubMed).

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998 (PubMed).

Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996 (PubMed).