Rabbit IgG polyclonal antibody for Hepatocyte growth factor ( HGF) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human HGF, different from the related mouse sequence by two amino acids.< br/>Immunogen was affinity purified.
At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Kilby, Afford, Li, Strain, Ahmed, Whittle: "Localisation of hepatocyte growth factor and its receptor (c-met) protein and mRNA in human term placenta." in: Growth factors (Chur, Switzerland), Vol. 13, Issue 1-2, pp. 133-9, (1996) (PubMed).
Tamura, Arakaki, Tsubouchi, Takada, Daikuhara: "Enhancement of human hepatocyte growth factor production by interleukin-1 alpha and -1 beta and tumor necrosis factor-alpha by fibroblasts in culture." in: The Journal of biological chemistry, Vol. 268, Issue 11, pp. 8140-5, (1993) (PubMed).
Gohda, Tsubouchi, Nakayama, Hirono, Sakiyama, Takahashi, Miyazaki, Hashimoto, Daikuhara: "Purification and partial characterization of hepatocyte growth factor from plasma of a patient with fulminant hepatic failure." in: The Journal of clinical investigation, Vol. 81, Issue 2, pp. 414-9, (1988) (PubMed).
Target
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Hepatocyte Growth Factor (HGF) is structurally similar, but not identical to scatter factor, a molecule shown to stimulate the dissociation and scattering of epithelial cells. It mapped to 7q21. 1 and involved in liver regeneration. Human HGF has been purified approximately 209,000-fold with 18 % yield from plasma of a patient with fulminant hepatic failure. It consists of 2 chains, heavy and light with molecular weights of 54,000-65,000 and 31,500-34,500, respectively. HGF is a potent mitogen, morphogen and motogen to both endothelial and epithelial cell types and is linked to a tyrosine kinase, proto-oncogene, c-met receptor. It indicated that HGF may serve as a paracrine mediator to control placental development and growth.