anti-Human TNFRSF12A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended TNFRSF12A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
  • CD266
  • FN14
  • TWEAKR
  • AI255180
  • C87282
  • Fn14
  • HPIP
  • TWEAK-R
  • TweakR
  • tumor necrosis factor receptor superfamily, member 12A
  • tumor necrosis factor receptor superfamily, member 12a
  • TNFRSF12A
  • Tnfrsf12a
Epitope
C-Term
15
11
4
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
93
68
30
3
3
2
2
2
2
1
1
1
Host
Rabbit
50
42
16
4
Clonality
Monoclonal
Conjugate
This TNFRSF12A antibody is un-conjugated
11
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
58
56
21
16
16
14
13
12
5
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1688736
$ 385.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1687984 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1688720 FACS IHC (p) WB Rabbit IgG C-Term Log in to see SP134
1 ABIN303304 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1687033 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN5554803 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN741069 IHC (p) HRP Rabbit IgG AA 101-129 Log in to see Polyclonal
1 ABIN1740476 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN741062 IHC (p) Biotin Rabbit IgG AA 101-129 Log in to see Polyclonal
1 ABIN741060 IF (p) IHC (p) Rabbit IgG AA 101-129 Log in to see Polyclonal
1 ABIN5554020 IHC (p) Rabbit IgG AA 55-63, Internal Region Log in to see Polyclonal
1 ABIN214532 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1942897 FACS IHC (p) WB Rabbit IgG C-Term Log in to see SP134
1 ABIN1942898 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN5589803 IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN5589804 IHC (p) Rabbit Log in to see Polyclonal
1 ABIN2766874 IHC (p) WB Rabbit Log in to see Polyclonal

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
Epitope C-Term
(15), (11), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(93), (68), (30), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(50), (42), (16), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF12A antibody is un-conjugated
(11), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(58), (56), (21), (16), (16), (14), (13), (12), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-TNFRSF12A Antibody

Target Details TNFRSF12A Application Details Handling Images
Predicted Reactivity Frog,Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Membrane
Purification Purified by Protein A/G
Immunogen Synthetic peptide near the C-terminus of human Fn14 protein.
Clone SP134
Isotype IgG

Target Details TNFRSF12A

Product Details anti-TNFRSF12A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Fn14 (TNFRSF12A Antibody Abstract)
Background Fn14/Tweak receptor/CD266/TNFRSF12A, tumor necrosis factor receptor superfamily member 12A, is the receptor for Tweak, a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins.
Molecular Weight 14-17 kDa
Gene ID 51330
Pathways Apoptosis, Regulation of Cell Size

Application Details

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Handling Images back to top
Application Notes IHC Dilution: Ready to use, for IHC only
Comment

Positive Control:
IHC (p): Renal cell carcinoma, kidney, lung adenocarcinoma
WB: Calu3 cell lysate
FC: HT29 Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue sections in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Images back to top
Format Liquid
Buffer pre-diluted rabbit monoclonal antibody purified by protein A/G in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) Flow cytometric analysis of rabbit anti-Fn14 (SP134) antibody in HT29 (green) compare...
Western Blotting (WB) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 11)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 12)
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (ABIN1688736) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) (C-Term) antibody (Image 13)