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|Application / Reactivity||Human|
|Blocking Reagent (BR)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||46 Antibodies|
|Functional Studies (Func)||14 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Mass Cytometry (CyTOF)||1 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Western Blotting (WB)||50 Antibodies|
|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies|
|Conjugate||This TNFRSF12A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-TNFRSF12A AntibodyTarget Details TNFRSF12A Application Details Handling References for anti-TNFRSF12A Antibody (ABIN499851) Images
|Specificity||This antibody reacts to Fn14.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Fn14 antibody was raised against a human IgG1 Fc-tagged Fn14 protein.|
Target Details TNFRSF12AProduct Details anti-TNFRSF12A Antibody Application Details Handling References for anti-TNFRSF12A Antibody (ABIN499851) Images back to top
|Alternative Name||CD266 / TweakR (TNFRSF12A Antibody Abstract)|
|Background||TWEAK is a TNF family member produced by IFN-gamma-stimulated monocytes and induces multiple cell death pathways including caspase-dependent apoptosis and cathepsin B-dependent necrosis. These pathways are mediated by the binding of TWEAK by fibroblast growth factor 14 (Fn14), a member of the TNF receptor superfamily. Yeast two-hybrid experiments have shown that FN14 will bind to the TNFR-associated factors TRAF1, TRAF2, TRAF3, and TRAF5, suggesting that the signaling pathway induced by TWEAK may be mediated by one or more of these adaptor molecules. Fn14 is induced during liver regeneration and is highly expressed in hepatocellular carcinomas. In addition, TWEAK induces liver progenitor cell proliferation, suggesting Fn14 may play a role in hepatocyte growth control and liver neoplasia.Synonyms: FGF-inducible 14, FN14, Fibroblast growth factor-inducible immediate-early response protein 14, TNFRSF12A, Tumor necrosis factor receptor superfamily member 12A, Tweak receptor|
Application DetailsProduct Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Handling References for anti-TNFRSF12A Antibody (ABIN499851) Images back to top
ELISA. Western BLot: 2-4 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details References for anti-TNFRSF12A Antibody (ABIN499851) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-TNFRSF12A Antibody (ABIN499851)Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Handling Images back to top
Brown, Richards, Hanscom et al.: "The Fn14 cytoplasmic tail binds tumour-necrosis-factor-receptor-associated factors 1, 2, 3 and 5 and mediates nuclear factor-kappaB activation." in: The Biochemical journal, Vol. 371, Issue Pt 2, pp. 395-403, 2003 (PubMed).
Nakayama, Ishidoh, Kojima et al.: "Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 341-8, 2002 (PubMed).
Feng, Guo, Factor et al.: "The Fn14 immediate-early response gene is induced during liver regeneration and highly expressed in both human and murine hepatocellular carcinomas." in: The American journal of pathology, Vol. 156, Issue 4, pp. 1253-61, 2000 (PubMed).
Chicheportiche, Bourdon, Xu et al.: "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32401-10, 1998 (PubMed).
ImagesProduct Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Handling References for anti-TNFRSF12A Antibody (ABIN499851) back to top