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anti-Mouse (Murine) TNFRSF12A antibody for ELISA

Recommended TNFRSF12A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
  • CD266
  • FN14
  • TWEAKR
  • AI255180
  • C87282
  • Fn14
  • HPIP
  • TWEAK-R
  • TweakR
  • tumor necrosis factor receptor superfamily, member 12A
  • tumor necrosis factor receptor superfamily, member 12a
  • TNFRSF12A
  • Tnfrsf12a
Epitope
Extracellular Domain
15
4
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
96
50
30
2
2
2
1
1
1
1
1
1
Host
Goat
51
45
2
1
Clonality
Polyclonal
Conjugate
This TNFRSF12A antibody is un-conjugated
10
6
4
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
56
44
20
16
15
13
12
10
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN956715
$ 595.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
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1 ABIN2142027 Func ELISA Mouse IgG2b kappa Log in to see ITEM-4
1 ABIN1856929 IHC ELISA WB Rabbit Log in to see Polyclonal

Similar anti-TNFRSF12A Antibodies

Application / Reactivity Mouse (Murine)
ELISA 6 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Functional Studies (Func) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Western Blotting (WB) 27 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
Epitope Extracellular Domain
(15), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(96), (50), (30), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Goat
(51), (45), (2), (1)
Clonality
Polyclonal
Conjugate This TNFRSF12A antibody is un-conjugated
(10), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(56), (44), (20), (16), (15), (13), (12), (10), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-TNFRSF12A Antibody

Target Details TNFRSF12A Application Details Handling Images
Specificity Mouse TWEAK R extracellular domain.
Purification Immunoaffinity purified
Sterility 0.2 μm filtered
Immunogen Purified, E. coli-derived, recombinant mouse TNF-related Weak inducer of Apoptosis Receptor (rmTWEAK R) extracellular domain.

Type of Immunogen: Recombinant protein
Isotype IgG

Target Details TNFRSF12A

Product Details anti-TNFRSF12A Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNFRSF12A / FN14 (TNFRSF12A Antibody Abstract)
Background Name/Gene ID: TNFRSF12A

Synonyms: TNFRSF12A, CD266 antigen, CD266, FGF-inducible 14, FN14, Tweak-receptor, TWEAKR
Gene ID 51330
UniProt Q9NP84
Pathways Apoptosis

Application Details

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Handling Images back to top
Application Notes Approved: ELISA (0.8 μg/mL), Flo (50 μg/mL), IHC, WB (0.1 - 0.2 μg/mL)

Usage: Western blot-This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse TWEAK R. The detection limit for rmTWEAK R is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry-This antibody will detect TWEAK R in cells and tissues. The working dilution is 5-15 μg/mL. Flow Cytometry-This antibody has been tested on MS-1 cells for use in flow cytometry. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 105 cells in a total reaction volume not exceeding 200 μL. The binding of. unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. ELISA Capture-This antibody can be used as a capture antibody in a mouse TWEAK R ELISA in combination with biotinylated, mouse TWEAK R affinity purified polyclonal detection antibody. A general protocol is provided on the next page. Using plates coated with 100 μL/well of the detection antibody, in combination with 100 μL/well of the capture antibody at 0.8 μg/mL, an ELISA for. sample volumes of 100 μL can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 20 ng/mL. In this format, this antibody shows less than 2 % cross-reactivity with rhTWEAKR.
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 200 μL sterile PBS.
Concentration Lot specific
Buffer Lyophilized from 0.2 μm filtered PBS, pH 7.4, 5 % trehalose
Handling Advice Avoid frequent freeze thaws. Store undiluted.
Storage -20 °C,-80 °C
Storage Comment Store at -20°C to -70°C. Stable for 12 months from date of receipt.