anti-Human PIAS1 antibody for Enzyme Immunoassay

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT 1
  • LOC100232805
  • Pias1
  • PIAS1
  • pias1
Epitope
C-Term
34
27
18
5
3
3
3
3
2
2
1
1
1
1
Reactivity
Human
90
37
20
5
4
3
3
3
3
2
2
2
1
1
Host
Rabbit
85
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
88
57
53
20
8
6
5
3
2
2
1
1
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Catalog No. ABIN5611025
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5555119 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN615743 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope C-Term
(34), (27), (18), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human
(90), (37), (20), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(85), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(88), (57), (53), (20), (8), (6), (5), (3), (2), (2), (1), (1)
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling Images
Specificity This antibody is specific to PIAS1 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIAS1.
Isotype Ig Fraction

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Gene ID 8554, 9606
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.