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|Antigen||Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies|
|Conjugate||This PIAS1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PIAS1 AntibodyTarget Details PIAS1 Application Details Handling Images
|Specificity||This antibody is specific to PIAS1 (C-term).|
|Purification||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIAS1.|
Target Details PIAS1Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
|Alternative Name||PIAS1 (PIAS1 Antibody Abstract)|
|Background||PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein|
|Gene ID||8554, 9606|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors|
|Pathways||JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C|
Application DetailsProduct Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|