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anti-Human PIAS1 antibody for Enzyme Immunoassay

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • DDXBP1
  • ddxbp1
  • Ddxbp1
  • GBP
  • gbp
  • GU/RH-II
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • ZMIZ3
Epitope
C-Term
34
28
17
5
3
3
3
2
2
2
1
1
1
1
Reactivity
Human
89
31
17
2
2
1
1
1
Host
Rabbit
84
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
82
53
52
15
8
7
6
2
2
2
1
1
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Catalog No. ABIN356763
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000887512 ABIN500489 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.000887512 ABIN615743 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PIAS1 antibody for Enzyme Immunoassay

Similar anti-PIAS1 Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope C-Term
(34), (28), (17), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(89), (31), (17), (2), (2), (1), (1), (1)
Host Rabbit
(84), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(82), (53), (52), (15), (8), (7), (6), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN356763) Images
Specificity This antibody is specific to PIAS1 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIAS1.
Isotype Ig

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling References for anti-PIAS1 Antibody (ABIN356763) Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Gene ID 8554, 9606
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling References for anti-PIAS1 Antibody (ABIN356763) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details References for anti-PIAS1 Antibody (ABIN356763) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PIAS1 Antibody (ABIN356763)

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Background publications

Miyauchi, Yogosawa, Honda, Nishida, Yasuda: "Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 50131-6, 2002

Nishida, Yasuda: "PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor and repress androgen receptor-dependent transcription." in: The Journal of biological chemistry, Vol. 277, Issue 44, pp. 41311-7, 2002

Tan, Hall, Hamil, Grossman, Petrusz, French: "Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators." in: The Journal of biological chemistry, Vol. 277, Issue 19, pp. 16993-7001, 2002

Megidish, Xu, Xu: "Activation of p53 by protein inhibitor of activated Stat1 (PIAS1)." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 8255-9, 2002

Liu, Shuai: "Induction of apoptosis by protein inhibitor of activated Stat1 through c-Jun NH2-terminal kinase activation." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36624-31, 2001

Images

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Supplier Images
 image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (C-Term) (ABIN356763) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (C-Term) antibody
 image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (C-Term) (ABIN356763) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (C-Term) antibody (Image 2)