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anti-Human PIAS1 antibody for Immunohistochemistry (Frozen Sections)

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • DDXBP1
  • ddxbp1
  • Ddxbp1
  • GBP
  • gbp
  • GU/RH-II
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • ZMIZ3
Epitope
AA 409-645
34
29
17
5
3
3
2
2
2
2
1
1
1
1
Reactivity
Human
89
31
17
2
2
1
1
1
Host
Rabbit
84
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
82
53
51
15
8
6
6
3
2
2
1
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Catalog No. ABIN1860208
$ 365.20
Plus shipping costs $45.00

Similar anti-PIAS1 Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope AA 409-645
(34), (29), (17), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(89), (31), (17), (2), (2), (1), (1), (1)
Host Rabbit
(84), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(82), (53), (51), (15), (8), (6), (6), (3), (2), (2), (1)
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ED GTWAPMRSKK EVQEVSASYN GVDGCLSSTL EHQVASHHQS SNKNKKVEVI DLTIDSSSDE EEEEPSAKRT CPSLSPTSPL NNKGILSLPH QASPVSRTPS LPAVDTSYIN TSLIQDYRHP FHMTPMPYDL QGLDFFPFLS GDNQHYNTSL LAAAAAAVSD DQDLLHSSRF FPYTSSQMFL DQLSAGGSTS LPTTNGSSSG SNSSLVSSNS LRESHSHTVT NRSSTDTASI FGIIP
Specificity It has been selected for its ability to recognize PIAS1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen PIAS1 (AA 409-645)
Isotype IgG

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Protein Inhibitor Of Activated STAT 1 (PIAS1) (PIAS1 Antibody Abstract)
Pathways JAK-STAT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant PIAS1 (Glu409~Pro645) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 409-645) (ABIN1860208) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 409-645) antibody