You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PIAS1 antibody for Western Blotting

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT 1
  • LOC100232805
  • Pias1
  • PIAS1
  • pias1
Epitope
N-Term
33
30
18
5
3
3
3
3
2
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
91
38
20
3
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
86
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
89
59
53
19
8
6
5
3
3
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2780997
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0977407 ABIN2780998 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0977407 ABIN500489 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
3.0977407 ABIN388055 IHC (p) WB Rabbit Ig AA 607-637, C-Term Log in to see Polyclonal 5
3.0977407 ABIN388054 IHC (p) WB Rabbit Ig AA 100-130, N-Term Log in to see Polyclonal 7
3.0977407 ABIN3044474 ICC IHC (p) WB Rabbit IgG AA 636-651, C-Term Log in to see Polyclonal
3.0977407 ABIN1533432 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN3186436 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
3.0977407 ABIN2957720 IHC WB Rabbit N-Term Log in to see Polyclonal
3.0977407 ABIN4345418 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.0977407 ABIN615743 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
3.0977407 ABIN407959 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN2499373 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN4345417 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
3.0977407 ABIN1860208 ICC IHC IP WB Rabbit IgG AA 409-645 Log in to see Polyclonal
3.0977407 ABIN2564529 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN2495457 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
3.0977407 ABIN2495456 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
3.0977407 ABIN1327703 WB Mouse AA 1-651, full length Log in to see Polyclonal
3.0977407 ABIN2432537 ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN522005 WB Mouse AA 1-651, full length Log in to see Polyclonal

Top referenced anti-PIAS1 antibody for Western Blotting

Similar anti-PIAS1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Xenopus laevis Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 87 Antibodies 38 Antibodies 21 Antibodies 2 Antibodies 4 Antibodies 3 Antibodies 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 6 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 13 Antibodies 8 Antibodies
Immunohistochemistry (IHC) 59 Antibodies 22 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope N-Term
(33), (30), (18), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(91), (38), (20), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(86), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Western Blotting (WB)
(89), (59), (53), (19), (8), (6), (5), (3), (3), (2), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN2780997) Images
Blocking Peptide Blocking peptide for this product available: ABIN981725
Sequence MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against PIAS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS1

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling References for anti-PIAS1 Antibody (ABIN2780997) Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.
Molecular Weight 72 kDa
Gene ID 8554
NCBI Accession NM_016166, NP_057250
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling References for anti-PIAS1 Antibody (ABIN2780997) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 651 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details References for anti-PIAS1 Antibody (ABIN2780997) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIAS1 Antibody (ABIN2780997)

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Background publications

Helbig, Yip, McCartney, Eyre, Beard: "A screening method for identifying disruptions in interferon signaling reveals HCV NS3/4a disrupts Stat-1 phosphorylation." in: Antiviral research, Vol. 77, Issue 3, pp. 169-76, 2008

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN2780997) back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (N-Term) (ABIN2780997) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (N-Term) antibody