anti-Mouse (Murine) PIAS1 antibody for Enzyme Immunoassay

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT 1
  • LOC100232805
  • Pias1
  • PIAS1
  • pias1
Epitope
C-Term
33
26
18
5
3
3
3
3
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
88
36
19
5
4
3
3
3
3
2
2
2
1
1
Host
Rabbit
83
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
86
57
52
18
7
6
5
3
2
1
1
1
Supplier
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Catalog No. ABIN5555119
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN615743 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Similar anti-PIAS1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 9 Antibodies 14 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 13 Antibodies
Immunoprecipitation (IP) 1 Antibodies 2 Antibodies
Western Blotting (WB) 20 Antibodies 36 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope C-Term
(33), (26), (18), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(88), (36), (19), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(83), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(86), (57), (52), (18), (7), (6), (5), (3), (2), (1), (1), (1)
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling Images
Specificity This antibody is predicted to not cross-react with other members of the PIAS family.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat.
Purification Affinity chromatography purified via peptide column
Immunogen 15 amino acid peptide near the carboxy terminus of Human PIAS1.
Isotype IgG

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated ?-globin expression in erythroid cells.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Gene ID 8554
NCBI Accession NP_057250
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
Application Notes ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.