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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||8 Antibodies||13 Antibodies||52 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies||20 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies||7 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||3 Antibodies|
|Western Blotting (WB)||17 Antibodies||31 Antibodies|
|Antigen||Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PIAS1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PIAS1 AntibodyTarget Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN500489) Images
|Specificity||This antibody is predicted to not cross-react with other members of the PIAS family.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse, Rat.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||15 amino acid peptide near the carboxy terminus of Human PIAS1.|
Target Details PIAS1Product Details anti-PIAS1 Antibody Application Details Handling References for anti-PIAS1 Antibody (ABIN500489) Images back to top
|Alternative Name||PIAS1 (PIAS1 Antibody Abstract)|
|Background||The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated ?-globin expression in erythroid cells.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors|
|Pathways||JAK-STAT Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-PIAS1 Antibody Target Details PIAS1 Handling References for anti-PIAS1 Antibody (ABIN500489) Images back to top
ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIAS1 Antibody Target Details PIAS1 Application Details References for anti-PIAS1 Antibody (ABIN500489) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-PIAS1 Antibody (ABIN500489)Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Kang, Chae, Jeon et al.: "PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific alpha-globin expression." in: Nucleic acids research, Vol. 38, Issue 16, pp. 5456-71, 2010 (PubMed).
Shuai, Liu: "Regulation of gene-activation pathways by PIAS proteins in the immune system." in: Nature reviews. Immunology, Vol. 5, Issue 8, pp. 593-605, 2005 (PubMed).
Liu, Liao, Rao et al.: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998 (PubMed).
ImagesProduct Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN500489) back to top