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anti-Rat (Rattus) STAT1 antibody for Enzyme Immunoassay

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
C-Term
94
78
69
27
23
20
18
18
14
12
12
11
7
6
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
556
241
178
29
17
16
15
14
7
5
4
3
2
2
2
2
2
2
1
1
Host
Rabbit
369
185
20
6
3
3
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
16
15
15
13
13
12
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
495
165
163
90
90
54
33
31
18
15
9
6
5
2
2
1
Supplier
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Catalog No. ABIN500815
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN401047 EIA WB Rabbit IgG pTyr701 Log in to see Polyclonal 1
0.0008867729 ABIN401411 EIA WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.0008867729 ABIN784192 EIA IHC (p) WB Rabbit IgG pSer727 Log in to see Polyclonal
0.0008867729 ABIN784212 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008867729 ABIN318124 EIA IHC (p) IP WB Rabbit pTyr701 Log in to see Polyclonal

Top referenced anti-STAT1 antibody for Enzyme Immunoassay

Similar anti-STAT1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 39 Antibodies 53 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies 7 Antibodies
Immunochromatography (IC) 2 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 34 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Western Blotting (WB) 135 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope C-Term
(94), (78), (69), (27), (23), (20), (18), (18), (14), (12), (12), (11), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(556), (241), (178), (29), (17), (16), (15), (14), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(369), (185), (20), (6), (3), (3), (1)
Clonality
Polyclonal
Conjugate This STAT1 antibody is un-conjugated
(16), (15), (15), (13), (13), (12), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(495), (165), (163), (90), (90), (54), (33), (31), (18), (15), (9), (6), (5), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN500815) Images
Specificity This antibody detects STAT1 alpha (C-term) at a 91 kDa band. No reaction to the 84 kDa STAT1a.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification DEAE purified
Immunogen STAT1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human STAT1 alpha. Remarks: The sequences differ from the murine corresponding sequences by four amino acids.
Isotype IgG

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN500815) Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Gene ID 6772
NCBI Accession NP_009330
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN500815) Images back to top
Application Notes ELISA. Western blot: 1: 1000 - 1: 2000. Immunoprecipitation: 2 - 4 μg. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN500815) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-STAT1 Antibody (ABIN500815)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Background publications

Leonard, OShea: "Jaks and STATs: biological implications." in: Annual review of immunology, Vol. 16, pp. 293-322, 1998

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997

Schindler, Darnell: "Transcriptional responses to polypeptide ligands: the JAK-STAT pathway." in: Annual review of biochemistry, Vol. 64, pp. 621-51, 1995

Schindler, Fu, Improta, Aebersold, Darnell: "Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7836-9, 1992

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN500815) back to top
Supplier Images
Immunofluorescence (IF) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term) (ABIN500815) Immunocytochemistry of STAT1 alpha in HeLa cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term) (ABIN500815) Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J),...