IL1B ELISA Kit (Interleukin 1, beta)

Details for Product IL1B ELISA Kit No. ABIN625019, Supplier: Log in to see
Antigen
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Alternatives
Chicken IL1B ELISA Kit
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
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48
38
17
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11
11
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3
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1
Method Type
Sandwich ELISA
Detection Range
0.3-100 pg/mL
Minimum Detection Limit
0.3 pg/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Cross-Reactivity (Details) This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF).
Sensitivity < 0.3 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Alternative Name IL-1 beta (IL1B ELISA Kit Abstract)
Background Monocytes are the main source of secreted IL-1. They express predominantly IL-1beta while human keratinocytes express large amounts of IL-1alpha. IL-1 is produced also by activated macrophages from different sources (alveolar macrophages, Kupffer cells, adherent spleen and peritoneal macrophages) and also by peripheral neutrophil granulocytes. IL-1alpha and IL-1 beta are biologically more or less equivalent pleiotropic factors that act locally and also systemically. IL-1beta is a potent immunomodulator, which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
Gene ID 3553
UniProt P01584
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation
  1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
    2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 2 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
    3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
    4. Preparation of standard: Briefly spin the vial of Item C and then add 880 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine) into Item C vial to prepare a 20 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 5 µL IL-1beta standard from the vial of Item C, into a tube with 995 µL Assay Diluent A or 1x Assay Diluent B to prepare a 100 pg/mL stock standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200myl 200 µL 200 µL 200 µL 200 µL 5 µL standard +995 µL 100 40 16 6.4 2.56 1.02 0.48 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
    5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
    6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
    7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 300-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 15 ml 1x Assay Diluent B to prepare a final 300 fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure
  1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
    2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
    3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
    4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
    5. Discard the solution. Repeat the wash as in step
    6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
    7. Discard the solution. Repeat the wash as in step
    8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
    9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results

Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Human IL-1beta concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Human IL-1beta concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of IL-1beta is typically less than 0.3 pg/mL.
Recovery: Recovery was determined by spiking various levels of human IL-1beta into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 98.87 89-108 Plasma 99.66 90-107 Cell culture media 100.43 89-110
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 97 99 98 Range ( %) 90-104 89-107 88-105 1:4 Average % of Expected 97 95 97 Range ( %) 89-107 88-105 91-107
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
ELISA image for Interleukin 1, beta (IL1B) ELISA Kit (ABIN625019) Interleukin 1, beta (IL1B) ELISA Kit
Product cited in: Yiu, Chan, Li, Tsang, Verdolini Abbott, Kwong, Ma, Tse, Lin: "Wound-healing effect of acupuncture for treating phonotraumatic vocal pathologies: A cytokine study." in: The Laryngoscope, Vol. 126, Issue 1, pp. E18-22, 2016 (PubMed).

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Ildefonso, Jaime, Rahman, Li, Boye, Hauswirth, Lucas, McFadden, Lewin: "Gene delivery of a viral anti-inflammatory protein to combat ocular inflammation." in: Human gene therapy, Vol. 26, Issue 1, pp. 59-68, 2015 (PubMed).

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Filková, Vernerová, Hulejová, Prajzlerová, Veigl, Pavelka, Vencovský, Šenolt: "Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis." in: Cytokine, Vol. 73, Issue 1, pp. 36-43, 2015 (PubMed).

Ildefonso, Jaime, Biswal, Boye, Li, Hauswirth, Lewin: "Gene therapy with the caspase activation and recruitment domain reduces the ocular inflammatory response." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 23, Issue 5, pp. 875-84, 2015 (PubMed).

Diabate, Munro, Garcia, Jacquel, Michel, Obba, Goncalves, Luci, Marchetti, Demon, Degos, Bechah, Mege, Lamkanfi, Auberger, Gorvel, Stuart, Landraud, Lemichez, Boyer: "Escherichia coli ?-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia." in: PLoS pathogens, Vol. 11, Issue 3, pp. e1004732, 2015 (PubMed).

Jiang, Ling, Zhang, Mao, Ma, Yin, Wang, Tang, Tan, Shi, Li, Ruan: "Altered fecal microbiota composition in patients with major depressive disorder." in: Brain, behavior, and immunity, Vol. 48, pp. 186-94, 2015 (PubMed).

Mankad, Dupuis, Smile, Roberts, Brian, Lui, Genore, Zaghloul, Iaboni, Marcon, Anagnostou: "A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism." in: Molecular autism, Vol. 6, pp. 18, 2015 (PubMed).

Ye, Xu, Lou, Jin, Miao, Ye, Xi: "Anti-inflammatory effects of hinokitiol on human corneal epithelial cells: an in vitro study." in: Eye (London, England), Vol. 29, Issue 7, pp. 964-71, 2015 (PubMed).

Zhang, Zhao, Wang, Huang, Wang, Zhao, Yang: "Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells." in: Journal of translational medicine, Vol. 13, pp. 178, 2015 (PubMed).

Ansari, Dutta, Veettil, Dutta, Iqbal, Kumar, Roy, Chikoti, Singh, Chandran: "Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses." in: PLoS pathogens, Vol. 11, Issue 7, pp. e1005019, 2015 (PubMed).

Pulikkotil, Nath: "Effects of curcumin on crevicular levels of IL-1β and CCL28 in experimental gingivitis." in: Australian dental journal, Vol. 60, Issue 3, pp. 317-27, 2015 (PubMed).

Zhang, Wang, Miao, Chen, Qiang, Cui, Jing, Guo: "Magnetic ferroferric oxide nanoparticles induce vascular endothelial cell dysfunction and inflammation by disturbing autophagy." in: Journal of hazardous materials, Vol. 304, pp. 186-95, 2015 (PubMed).

Wu, Zheng, Jin, Zhang, He, Yang, Wen: "Chinese medicine Tongxinluo reduces atherosclerotic lesion by attenuating oxidative stress and inflammation in microvascular endothelial cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6323-33, 2015 (PubMed).

Scherer, Steinmetz, Kaether, Weinigel, Barz, Kleinert, Menche, Müller, Pergola, Werz: "Targeting V-ATPase in primary human monocytes by archazolid potently represses the classical secretion of cytokines due to accumulation at the endoplasmic reticulum." in: Biochemical pharmacology, Vol. 91, Issue 4, pp. 490-500, 2014 (PubMed).

Alkhatib, Rosenzweig, Krock, Roughley, Beckman, Steffen, Weber, Ouellet, Haglund: "Acute mechanical injury of the human intervertebral disc: link to degeneration and pain." in: European cells & materials, Vol. 28, pp. 98-110; discussion 110-1, 2014 (PubMed).

Chowdhury, Ahmed, Choudhuri, Sen, Hazra, Pal, Bhattacharya, Bahar: "Alteration of serum inflammatory cytokines in active pulmonary tuberculosis following anti-tuberculosis drug therapy." in: Molecular immunology, Vol. 62, Issue 1, pp. 159-68, 2014 (PubMed).

Duan, Yu, Yu, Li, Wang, Geng, Jiang, Li, Zhou, Sun: "Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway." in: International journal of nanomedicine, Vol. 9, pp. 5131-41, 2014 (PubMed).

Ertugrul, Sahin, Dikilitas, Alpaslan, Bozoglan: "Comparison of CCL28, interleukin-8, interleukin-1? and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis." in: Journal of periodontal research, Vol. 48, Issue 1, pp. 44-51, 2013 (PubMed).

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