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anti-Human MAP2K3 antibody for Western Blotting

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
  • MAP kinase activator XMEK3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 6
  • map2k3
  • Map2k3
  • MAP2K3
  • map2k6
Epitope
C-Term
31
24
15
14
14
11
11
11
7
7
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
297
149
141
56
40
5
5
4
4
2
2
2
1
1
1
Host
Rabbit
194
103
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
258
99
96
76
56
26
19
15
8
3
3
1
1
1
Options
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Catalog No. ABIN2777129
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0887618 ABIN2777128 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.0887618 ABIN1532059 IHC ELISA WB Rabbit IgG pThr222 Log in to see Polyclonal 1
3.0887618 ABIN1533090 ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.0887618 ABIN3043874 IHC (p) WB Rabbit IgG AA 311-347, C-Term Log in to see Polyclonal
3.0887618 ABIN3044392 IHC (p) WB Rabbit IgG AA 320-334, C-Term Log in to see Polyclonal
3.0887618 ABIN126840 EIA WB Mouse IgG1 Log in to see 5F7
3.0887618 ABIN658362 WB Rabbit Ig AA 132-160, Center Log in to see Polyclonal
3.0887618 ABIN1532939 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0887618 ABIN1870347 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
3.0887618 ABIN3185527 ELISA WB Rabbit IgG Thr383 Log in to see Polyclonal
3.0887618 ABIN3182069 ELISA IHC WB Rabbit IgG pSer218 Log in to see Polyclonal
3.0887618 ABIN499014 WB Rabbit Log in to see Polyclonal
3.0887618 ABIN257414 ICC IF IHC (p) IHC WB Rabbit pSer189 Log in to see Polyclonal
3.0887618 ABIN1873605 WB Rabbit IgG Log in to see Polyclonal
3.0887618 ABIN2737130 IHC WB Rabbit IgG Log in to see Polyclonal
3.0887618 ABIN407737 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
3.0887618 ABIN1859745 ICC IHC IP WB Rabbit IgG AA 99-343 Log in to see Polyclonal
3.0887618 ABIN519260 ELISA WB Mouse IgG1 kappa AA 1-100, partial Log in to see 2F12
3.0887618 ABIN519257 WB Mouse AA 1-347, full length Log in to see Polyclonal
3.0887618 ABIN631257 WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-MAP2K3 antibody for Western Blotting

Similar anti-MAP2K3 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Dog (Canine) Cow (Bovine) Horse (Equine) Rabbit Guinea Pig Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 258 Antibodies 57 Antibodies 122 Antibodies 111 Antibodies 6 Antibodies 5 Antibodies 6 Antibodies 3 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 15 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies 34 Antibodies 33 Antibodies 14 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Epitope C-Term
(31), (24), (15), (14), (14), (11), (11), (11), (7), (7), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(297), (149), (141), (56), (40), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(194), (103)
Clonality
Polyclonal
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(258), (99), (96), (76), (56), (26), (19), (15), (8), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777129) Images
Blocking Peptide Blocking peptide for this product available: ABIN979769
Sequence EEPSPQLPAD RFSPEFVDFT AQCLRKNPAE RMSYLELMEH PFFTLHKTKK
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against MAP2K3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K3

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777129) Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Alias Symbols: MAPKK3, MEK3, MKK3, PRKMK3, SAPKK2, SAPKK-2
Protein Interaction Partner: UBC, EGFR, MAPK14, MAP3K5, ARRB1, PKN1, ALDOC, APP, LRRK2, MAPK8IP2, MAPK8IP3, MAPK8IP1, SPAG9, MAP3K2, MAP2K3, PPP2R4, MAP3K4, MAP3K3, TINF2, MYC, RPL13, TAOK2, TAOK1, DCTN1, DYRK1B, PLCB2, SMAD7, MAPK12, MAPK3, ELK1, MAP2K6, NPHS1,
Protein Size: 318
Molecular Weight 36 kDa
Gene ID 5606
NCBI Accession NM_002756, NP_002747
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 Antibody (ABIN2777129) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 318 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 Antibody (ABIN2777129) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP2K3 Antibody (ABIN2777129)

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
Background publications

Prickett, Brautigan: "Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4217-27, 2007

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777129) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (C-Term) (ABIN2777129) WB Suggested Anti-MAP2K3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: