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anti-Human MAP2K3 antibody for Western Blotting

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • AW212142
  • map2k3
  • MAPKK3
  • MEK3
  • Mkk3
  • MKK3
  • mMKK3b
  • Prkmk3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • zMKK3
Epitope
C-Term
33
24
15
14
14
12
12
10
6
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
300
114
113
27
17
3
1
Host
Rabbit
199
103
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
255
102
88
79
47
24
21
13
8
3
1
1
1
Supplier
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Catalog No. ABIN2777129
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12599558 ABIN2777128 WB Rabbit N-Term Log in to see Polyclonal 1
0.12599558 ABIN1532059 IHC ELISA WB Rabbit IgG pThr222 Log in to see Polyclonal 1
0.12599558 ABIN1533090 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12599558 ABIN126840 EIA WB Mouse IgG1 Log in to see 5F7
0.12599558 ABIN3043874 IHC (p) WB Rabbit IgG AA 311-347, C-Term Log in to see Polyclonal
0.12599558 ABIN1532939 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12599558 ABIN658362 WB Rabbit Ig AA 132-160, Center Log in to see Polyclonal
0.12599558 ABIN3044392 WB Rabbit IgG AA 42-58, N-Term Log in to see Polyclonal
0.12599558 ABIN257414 ICC IF IHC (p) IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
0.12599558 ABIN499014 WB Rabbit Log in to see Polyclonal
0.12599558 ABIN1870347 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
0.12599558 ABIN2449377 IF IHC WB Rabbit IgG Ser189 Log in to see Polyclonal
0.12599558 ABIN2449378 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
0.12599558 ABIN2448589 ELISA WB Rabbit IgG Thr222 Log in to see Polyclonal
0.12599558 ABIN2448593 IHC ELISA WB Rabbit IgG pThr222 Log in to see Polyclonal
0.12599558 ABIN2449365 IHC ELISA WB Rabbit IgG pSer189 Log in to see Polyclonal
0.12599558 ABIN2449364 IHC ELISA WB Rabbit IgG Ser189 Log in to see Polyclonal
0.12599558 ABIN1873605 WB Rabbit IgG Log in to see Polyclonal
0.12599558 ABIN519257 WB Mouse AA 1-347, full length Log in to see Polyclonal
0.12599558 ABIN519260 ELISA WB Mouse IgG1 kappa AA 1-100, partial Log in to see 2F12

Top referenced anti-MAP2K3 antibody for Western Blotting

Similar anti-MAP2K3 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus) Cow (Bovine) Zebrafish (Danio rerio) Chicken Xenopus laevis
Western Blotting (WB) 254 Antibodies 28 Antibodies 88 Antibodies 87 Antibodies 2 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 13 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies 16 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 88 Antibodies 53 Antibodies 41 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Epitope C-Term
(33), (24), (15), (14), (14), (12), (12), (10), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(300), (114), (113), (27), (17), (3), (1)
Host Rabbit
(199), (103)
Clonality
Polyclonal
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(255), (102), (88), (79), (47), (24), (21), (13), (8), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777129) Images
Blocking Peptide Blocking peptide for this product available: ABIN979769
Sequence EEPSPQLPAD RFSPEFVDFT AQCLRKNPAE RMSYLELMEH PFFTLHKTKK
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Rabbit : 100 %, Mouse : 90 %, Rat : 90 %, African clawed frog : 80 %, Chicken : 70 %
Characteristics This is a rabbit polyclonal antibody against MAP2K3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K3

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777129) Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Molecular Weight 36 kDa
Gene ID 5606
NCBI Accession NP_002747, NM_002756
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 Antibody (ABIN2777129) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 318 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 Antibody (ABIN2777129) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP2K3 Antibody (ABIN2777129)

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
Background publications

Prickett, Brautigan: "Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4217-27, 2007

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777129) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (C-Term) (ABIN2777129) WB Suggested Anti-MAP2K3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: