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anti-Human MAP2K3 antibody for Western Blotting

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
  • MAP kinase activator XMEK3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 6
  • map2k3
  • Map2k3
  • MAP2K3
  • map2k6
Epitope
pThr222
33
24
15
14
14
12
12
11
7
7
5
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
304
153
147
57
40
6
4
4
3
3
3
1
1
1
1
Host
Rabbit
201
103
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
265
102
100
78
56
26
19
14
8
3
3
1
1
1
Options
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Catalog No. ABIN1532059
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN2777128 WB Rabbit N-Term Log in to see Polyclonal 1
2.9590726 ABIN2777129 WB Rabbit C-Term Log in to see Polyclonal 1
2.9590726 ABIN1533090 ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.9590726 ABIN3043874 IHC (p) WB Rabbit IgG AA 311-347, C-Term Log in to see Polyclonal
2.9590726 ABIN126840 EIA WB Mouse IgG1 Log in to see 5F7
2.9590726 ABIN3044392 WB Rabbit IgG AA 42-58, N-Term Log in to see Polyclonal
2.9590726 ABIN658362 WB Rabbit Ig AA 132-160, Center Log in to see Polyclonal
2.9590726 ABIN1532939 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN1870347 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
2.9590726 ABIN3185527 ELISA WB Rabbit IgG Thr383 Log in to see Polyclonal
2.9590726 ABIN3182069 ELISA IHC WB Rabbit IgG pSer218 Log in to see Polyclonal
2.9590726 ABIN499014 WB Rabbit Log in to see Polyclonal
2.9590726 ABIN257414 ICC IF IHC (p) IHC WB Rabbit pSer189 Log in to see Polyclonal
2.9590726 ABIN2449378 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
2.9590726 ABIN2449377 IF IHC WB Rabbit IgG Ser189 Log in to see Polyclonal
2.9590726 ABIN1873605 WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN407737 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN2449365 IHC ELISA WB Rabbit IgG pSer189 Log in to see Polyclonal
2.9590726 ABIN2449364 IHC ELISA WB Rabbit IgG Ser189 Log in to see Polyclonal
2.9590726 ABIN2737130 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAP2K3 antibody for Western Blotting

Similar anti-MAP2K3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 36 Antibodies 44 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 1 Antibodies
Immunofluorescence (IF) 36 Antibodies 36 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies 26 Antibodies
Immunohistochemistry (IHC) 57 Antibodies 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 33 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 117 Antibodies 126 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Epitope pThr222
(33), (24), (15), (14), (14), (12), (12), (11), (7), (7), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(304), (153), (147), (57), (40), (6), (4), (4), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(201), (103)
Clonality
Polyclonal
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(265), (102), (100), (78), (56), (26), (19), (14), (8), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN1532059) Images
Specificity MAP2K3 (Phospho-Thr222) Antibody detects endogenous levels of MAP2K3 only when phosphorylated at Thr222.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human MAP2K3 around the phosphorylation site of Thr222.
Isotype IgG

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 Antibody (ABIN1532059) Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background Molecular weight of target protein: 39 kDa
Synonyms: dual specificity mitogen-activated protein kinase kinase 3, kinase MKK3, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3, MP2K3, PRKMK3
Molecular Weight 39 kDa
Gene ID 5606
OMIM 602315
UniProt P46734
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 Antibody (ABIN1532059) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.514012 (NCBI Gene Symbol: MAP2K3)

Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 Antibody (ABIN1532059) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MAP2K3 Antibody (ABIN1532059)

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
Background publications

Dérijard, Raingeaud, Barrett, Wu, Han, Ulevitch, Davis: "Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms." in: Science (New York, N.Y.), Vol. 267, Issue 5198, pp. 682-5, 1995

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN1532059) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pThr222) (ABIN1532059) Western blot analysis of extracts from Jurkat cells treated with serum 20% 15', using...
Immunohistochemistry (IHC) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pThr222) (ABIN1532059) Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho...