|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||21 Antibodies||25 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies||19 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||7 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||42 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K7 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-MAP2K7 AntibodyTarget Details MAP2K7 Application Details Handling Images
|Specificity||This antibody specifically recognizes the N-terminus of MKK7.|
|Characteristics||Synonyms: MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7,NKK2, MEK7, MKK7, MAP kinase kinase 7, Dual specificity mitogen-activated protein kinasekinase 7|
|Purification||Size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH|
Target Details MAP2K7Product Details anti-MAP2K7 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K7 (MAP2K7 Antibody Abstract)|
|Background||The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.Synonyms: Dual specificity mitogen-activated protein kinase kinase 7, JNK-activating kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK7, MKK7, NKK2, c-Jun N-terminal kinase kinase 2|
|Molecular Weight||45 kDa|
|Pathways||MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-MAP2K7 Antibody Target Details MAP2K7 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated SW480 cells. ELISA: 0.1 μg/mL. Immunocytochemistry. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Handling back to top