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anti-Mouse (Murine) IL1R1 antibody for Blocking Reagent

Recommended IL1R1 Antibody (supplied by: Log in to see )

Antigen
Interleukin 1 Receptor, Type I (IL1R1) Antibodies
  • CD121a
  • CD121A
  • CD121b
  • D2S1473
  • IL-1R-alpha
  • IL-iR
  • IL1R
  • Il1r-1
  • IL1RA
  • P80
Reactivity
Mouse (Murine)
106
60
24
2
1
Host
Rat
108
31
24
18
2
Clonality
Monoclonal
Conjugate
This IL1R1 antibody is un-conjugated
12
10
8
7
4
4
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
104
97
35
34
26
15
15
7
7
5
5
4
3
2
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1176949
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008700774 ABIN2664176 BR FACS IF Hamster IgG Log in to see JAMA-147 3

Top referenced anti-IL1R1 antibody for Blocking Reagent

Similar anti-IL1R1 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies
ELISA 15 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunodiffusion (ID) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Inhibition Assay (InhA) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Neutralization (Neut) 4 Antibodies
Purification (Purif) 2 Antibodies
Screening Assay (ScA) 1 Antibodies
Western Blotting (WB) 32 Antibodies

General

Antigen Interleukin 1 Receptor, Type I (IL1R1) Antibodies
Reactivity Mouse (Murine)
(106), (60), (24), (2), (1)
Host Rat
(108), (31), (24), (18), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1R1 antibody is un-conjugated
(12), (10), (8), (7), (4), (4), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(104), (97), (35), (34), (26), (15), (15), (7), (7), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL1R1 Antibody

Target Details IL1R1 Application Details Handling References for anti-IL1R1 Antibody (ABIN1176949) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
Clone 35F5
Isotype IgG1, kappa

Target Details IL1R1

Product Details anti-IL1R1 Antibody Application Details Handling References for anti-IL1R1 Antibody (ABIN1176949) Images back to top
Antigen
Alternative Name CD121a (IL1R1 Antibody Abstract)
Background The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
Synonyms: IL-1 Receptor, Type I/p80
Pathways NF-kappaB Signaling

Application Details

Product Details anti-IL1R1 Antibody Target Details IL1R1 Handling References for anti-IL1R1 Antibody (ABIN1176949) Images back to top
Application Notes Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.
Restrictions For Research Use only

Handling

Product Details anti-IL1R1 Antibody Target Details IL1R1 Application Details References for anti-IL1R1 Antibody (ABIN1176949) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL1R1 Antibody (ABIN1176949)

Product Details anti-IL1R1 Antibody Target Details IL1R1 Application Details Handling Images back to top
Product cited in:

McIntyre, Stepan, Kolinsky, Benjamin, Plocinski, Kaffka, Campen, Chizzonite, Kilian: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." in: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, 1991

Chizzonite, Truitt, Kilian, Stern, Nunes, Parker, Kaffka, Chua, Lugg, Gubler: "Two high-affinity interleukin 1 receptors represent separate gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, 1989