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anti-Human IRAK1 antibody for Western Blotting

Recommended IRAK1 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
  • AA408924
  • Il1rak
  • IRAK
  • IRAK-1
  • IRAK1
  • IRAK1-S
  • mPLK
  • pelle
  • Plpk
  • RGD1563841
Epitope
AA 325-360
40
37
22
22
18
15
14
13
13
13
12
9
8
7
7
6
5
5
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
238
110
77
Host
Rabbit
221
46
2
Clonality
Polyclonal
Conjugate
This IRAK1 antibody is un-conjugated
13
11
10
6
6
6
4
4
4
4
4
4
4
4
4
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
191
124
69
43
43
40
29
19
16
13
9
3
2
Supplier
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Available images

Catalog No. ABIN1387749
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1531673 ELISA WB Rabbit IgG pThr209 Log in to see Polyclonal 1
0.12303223 ABIN1531672 IHC ELISA WB Rabbit IgG pThr100 Log in to see Polyclonal 1
0.12303223 ABIN392233 IHC (p) WB Rabbit Ig AA 683-712, C-Term Log in to see Polyclonal 3
0.12303223 ABIN393660 IF WB Mouse IgG1 kappa AA 530-693 Log in to see 3A9 5
0.12303223 ABIN393719 IF WB Mouse IgG2a kappa AA 530-693 Log in to see 3F7 5
0.12303223 ABIN500036 EIA IF IP WB Rabbit IgG C-Term Log in to see Polyclonal 5
0.12303223 ABIN492547 EIA IHC (p) WB Mouse IgG AA 618-712 Log in to see 1F12C1
0.12303223 ABIN492548 EIA IHC (p) WB Mouse IgG AA 618-712 Log in to see 8F1A7
0.12303223 ABIN1532349 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1532697 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN3042847 WB Rabbit IgG AA 18-37, N-Term Log in to see Polyclonal
0.12303223 ABIN498209 IHC (p) WB Rabbit Log in to see Polyclonal
0.12303223 ABIN742658 IF (p) IHC (p) WB Rabbit IgG AA 170-220, pThr209 Log in to see Polyclonal
0.12303223 ABIN614624 EIA IF IHC (p) WB Mouse IgG2a AA 530-693 Log in to see 3F7
0.12303223 ABIN614625 EIA IF IHC (p) IP WB Mouse IgG1 AA 530-693 Log in to see 3A9
0.12303223 ABIN2706406 WB Rabbit pSer209 Log in to see Polyclonal
0.12303223 ABIN615177 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2650927 WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2490439 ELISA WB Rabbit IgG pThr209 Log in to see Polyclonal
0.12303223 ABIN2490740 IHC ELISA WB Rabbit IgG pThr100 Log in to see Polyclonal

Top referenced anti-IRAK1 antibody for Western Blotting

Similar anti-IRAK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 2 Antibodies 2 Antibodies
ELISA 16 Antibodies 42 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 17 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 33 Antibodies
Flow Cytometry (FACS) 16 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
Epitope AA 325-360
(40), (37), (22), (22), (18), (15), (14), (13), (13), (13), (12), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(238), (110), (77)
Host Rabbit
(221), (46), (2)
Clonality
Polyclonal
Conjugate This IRAK1 antibody is un-conjugated
(13), (11), (10), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(191), (124), (69), (43), (43), (40), (29), (19), (16), (13), (9), (3), (2)
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Product Details anti-IRAK1 Antibody

Target Details IRAK1 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human IRAK1
Isotype IgG

Target Details IRAK1

Product Details anti-IRAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IRAK1 (IRAK1 Antibody Abstract)
Background

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Subcellular location: Cytoplasm, Nucleus


Synonyms: IRAK, pelle, Interleukin-1 receptor-associated kinase 1, IRAK-1, IRAK1
Gene ID 3654
HGNC 0
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-IRAK1 Antibody Target Details IRAK1 Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (AA 325-360) (ABIN1387749) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-IRAK1 Polyclo...
'Independent Validation' Badge
Lot Number 140120
Method validated Western Blotting
Positive Control PC3 cells
Negative Control C6/36 cells (non-reactive species)
Notes A band was observed in the positive control sample at the correct molecular weight, which was absent from the negative control sample. Additional bands were also observed in the positive control sample, which were absent from the negative control. These bands may represent alternative IRAK1 isoforms.
Primary Antibody
  • Antigen: Interleukin-1 Receptor-Associated Kinase 1 (IRAK1)
  • Catalog number: ABIN1387749
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  • Supplier catalog number: Log in to see
  • Lot number: 140120
  • Antibody Dilution: 1:100
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
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  • Catalog number: Log in to see
  • Lot number: L170-6515
  • Antibody Dilution: 1:10,000
Controls
  • Positive control: PC3 cells
  • Negative control: C6/36 cells
Protocol
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 24 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (AA 325-360) antibody (ABIN1387749) Figure 1: Western Blot for IRAK1. Arrowhead indicates the expected molecular weight o...