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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||23 Antibodies||44 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies||5 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies||8 Antibodies|
|Immunofluorescence (IF)||10 Antibodies||12 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||52 Antibodies||52 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies||20 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies|
|Western Blotting (WB)||43 Antibodies|
|Flow Cytometry (FACS)||16 Antibodies|
|Antigen||Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This IRAK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-IRAK1 AntibodyTarget Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN401038) Images
|Specificity||This IgG fraction antibody is directed against Human IRAK protein. A BLAST analysis was used to suggest cross reactivity with IRAK protein from Human, Mouse and Rat based on 100 % homology with the immunizing sequence. No Cross reactivity occurs against IRAK-2. Reactivity against homologues from other sources is not known.|
Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to a region near the carboxy terminus of Human IRAK protein.|
Target Details IRAK1Product Details anti-IRAK1 Antibody Application Details Handling References for anti-IRAK1 Antibody (ABIN401038) Images back to top
|Alternative Name||IRAK1 (IRAK1 Antibody Abstract)|
|Background||IRAK (Interleukin-1 receptor-associated kinase 1). Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1|
|Research Area||Cytokines, Receptors, Inflammation|
|Pathways||NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-IRAK1 Antibody Target Details IRAK1 Handling References for anti-IRAK1 Antibody (ABIN401038) Images back to top
ELISA: 1/5000 - 1/20000. Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/500. Immunoprecipitation: 2-4 μg per sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IRAK1 Antibody Target Details IRAK1 Application Details References for anti-IRAK1 Antibody (ABIN401038) Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IRAK1 Antibody (ABIN401038)Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling Images back to top
Yao, Li, Shi: "Optically active supramolecular complexes of water-soluble achiral polythiophenes and folic acid: spectroscopic studies and sensing applications." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 24, Issue 22, pp. 12829-35, 2008
Huang, Gao, Li, Cao: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998
Robinson, Shibuya, Mui, Zonin, Murphy, Sana, Hartley, Menon, Kastelein, Bazan, OGarra: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997
Trofimova, Sprenkle, Green, Sturgill, Goebl, Harrington: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996
Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996
ImagesProduct Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN401038) back to top