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anti-Mouse (Murine) IRAK1 antibody for Enzyme Immunoassay

Recommended IRAK1 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
  • AA408924
  • Il1rak
  • IRAK
  • IRAK-1
  • IRAK1
  • IRAK1-S
  • mPLK
  • pelle
  • Plpk
  • RGD1563841
Epitope
C-Term
39
37
22
22
18
15
14
13
13
13
13
9
8
7
7
6
5
5
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
238
110
77
Host
Rabbit
221
46
2
Clonality
Polyclonal
Conjugate
This IRAK1 antibody is un-conjugated
13
11
10
6
6
6
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
191
124
69
44
43
39
28
19
16
13
8
3
2
Supplier
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Catalog No. ABIN401038
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008855868 ABIN500036 EIA IF IP WB Rabbit IgG C-Term Log in to see Polyclonal 5
0.0008855868 ABIN492547 EIA IHC (p) WB Mouse IgG AA 618-712 Log in to see 1F12C1
0.0008855868 ABIN492548 EIA IHC (p) WB Mouse IgG AA 618-712 Log in to see 8F1A7
0.0008855868 ABIN303104 EIA IHC (p) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-IRAK1 antibody for Enzyme Immunoassay

Similar anti-IRAK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 2 Antibodies 2 Antibodies
ELISA 16 Antibodies 42 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 17 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 33 Antibodies
Flow Cytometry (FACS) 16 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
Epitope C-Term
(39), (37), (22), (22), (18), (15), (14), (13), (13), (13), (13), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(238), (110), (77)
Host Rabbit
(221), (46), (2)
Clonality
Polyclonal
Conjugate This IRAK1 antibody is un-conjugated
(13), (11), (10), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(191), (124), (69), (44), (43), (39), (28), (19), (16), (13), (8), (3), (2)
Pubmed 5 references available
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Product Details anti-IRAK1 Antibody

Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN401038) Images
Specificity This IgG fraction antibody is directed against Human IRAK protein. A BLAST analysis was used to suggest cross reactivity with IRAK protein from Human, Mouse and Rat based on 100 % homology with the immunizing sequence. No Cross reactivity occurs against IRAK-2. Reactivity against homologues from other sources is not known.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
Purification DEAE Chromatography
Immunogen Synthetic peptide corresponding to a region near the carboxy terminus of Human IRAK protein.
Isotype IgG

Target Details IRAK1

Product Details anti-IRAK1 Antibody Application Details Handling References for anti-IRAK1 Antibody (ABIN401038) Images back to top
Antigen
Alternative Name IRAK1 (IRAK1 Antibody Abstract)
Background IRAK (Interleukin-1 receptor-associated kinase 1). Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1
Gene ID 3654
NCBI Accession NP_001020413
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-IRAK1 Antibody Target Details IRAK1 Handling References for anti-IRAK1 Antibody (ABIN401038) Images back to top
Application Notes ELISA: 1/5000 - 1/20000. Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/500. Immunoprecipitation: 2-4 μg per sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details References for anti-IRAK1 Antibody (ABIN401038) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-IRAK1 Antibody (ABIN401038)

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling Images back to top
Background publications

Yao, Li, Shi: "Optically active supramolecular complexes of water-soluble achiral polythiophenes and folic acid: spectroscopic studies and sensing applications." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 24, Issue 22, pp. 12829-35, 2008

Huang, Gao, Li, Cao: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998

Robinson, Shibuya, Mui, Zonin, Murphy, Sana, Hartley, Menon, Kastelein, Bazan, OGarra: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997

Trofimova, Sprenkle, Green, Sturgill, Goebl, Harrington: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996

Images

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN401038) back to top
Supplier Images
 image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN401038) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody
 image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN401038) anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody (Image 2)