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anti-Human MAP3K14 antibody for Enzyme Immunoassay

Recommended MAP3K14 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 14 (MAP3K14) Antibodies
  • aly
  • F23F1.4
  • F23F1_4
  • FTDCR1B
  • HS
  • HSNIK
  • mitogen-activated protein kinase kinase kinase 14
  • NIK
  • Nik
Epitope
Middle Region
37
19
15
13
8
6
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
71
55
21
1
1
Host
Rabbit
85
2
Clonality
Polyclonal
Conjugate
This MAP3K14 antibody is un-conjugated
5
5
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
73
33
31
23
22
21
11
1
1
1
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Catalog No. ABIN360384
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN500347 EIA WB Rabbit IgG AA 931-947, C-Term Log in to see Polyclonal 1

Top referenced anti-MAP3K14 antibody for Enzyme Immunoassay

Similar anti-MAP3K14 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 5 Antibodies 17 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 24 Antibodies
Western Blotting (WB) 11 Antibodies 59 Antibodies
Immunofluorescence (IF) 22 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 14 (MAP3K14) Antibodies
Epitope Middle Region
(37), (19), (15), (13), (8), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(71), (55), (21), (1), (1)
Host Rabbit
(85), (2)
Clonality
Polyclonal
Conjugate This MAP3K14 antibody is un-conjugated
(5), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(73), (33), (31), (23), (22), (21), (11), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-MAP3K14 Antibody

Target Details MAP3K14 Application Details Handling References for anti-MAP3K14 Antibody (ABIN360384) Images
Specificity This antibody reacts to NFkB Inducing Kinase (NIK).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NIK.
Isotype Ig

Target Details MAP3K14

Product Details anti-MAP3K14 Antibody Application Details Handling References for anti-MAP3K14 Antibody (ABIN360384) Images back to top
Antigen
Alternative Name MAP3K14 (MAP3K14 Antibody Abstract)
Background NIK (mitogen-activated protein kinase kinase kinase 14), a member of the Ser/Thr protein kinase family, binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.Synonyms: Mitogen-activated protein kinase kinase kinase 14, NF-kappa beta-inducing kinase, Serine/threonine-protein kinase NIK
Gene ID 9020
NCBI Accession NP_003945
UniProt Q99558
Research Area Kinases/Phosphatases, Transcription Factors
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K14 Antibody Target Details MAP3K14 Handling References for anti-MAP3K14 Antibody (ABIN360384) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K14 Antibody Target Details MAP3K14 Application Details References for anti-MAP3K14 Antibody (ABIN360384) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP3K14 Antibody (ABIN360384)

Product Details anti-MAP3K14 Antibody Target Details MAP3K14 Application Details Handling Images back to top
Background publications

Andreakos, Smith, Monaco, Brennan, Foxwell, Feldmann: "Ikappa B kinase 2 but not NF-kappa B-inducing kinase is essential for effective DC antigen presentation in the allogeneic mixed lymphocyte reaction." in: Blood, Vol. 101, Issue 3, pp. 983-91, 2003

Ninomiya-Tsuji, Kishimoto, Hiyama, Inoue, Cao, Matsumoto: "The kinase TAK1 can activate the NIK-I kappaB as well as the MAP kinase cascade in the IL-1 signalling pathway." in: Nature, Vol. 398, Issue 6724, pp. 252-6, 1999

Aronsson, Magnusson, Andersson, Karsten, Shibasaki, Lendon, Goate, Brookes: "The NIK protein kinase and C17orf1 genes: chromosomal mapping, gene structures and mutational screening in frontotemporal dementia and parkinsonism linked to chromosome 17." in: Human genetics, Vol. 103, Issue 3, pp. 340-5, 1998

Images

Product Details anti-MAP3K14 Antibody Target Details MAP3K14 Application Details Handling References for anti-MAP3K14 Antibody (ABIN360384) back to top
Supplier Images
 image for anti-MAP3K14 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 14) (Middle Region) (ABIN360384) anti-Mitogen-Activated Protein Kinase Kinase Kinase 14 (MAP3K14) (Middle Region) antibody
 image for anti-MAP3K14 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 14) (Middle Region) (ABIN360384) anti-Mitogen-Activated Protein Kinase Kinase Kinase 14 (MAP3K14) (Middle Region) antibody (Image 2)