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anti-Human TNFRSF11A antibody for Western Blotting

Recommended TNFRSF11A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) Antibodies
  • RANK
  • RGD1563614
  • TNFRSF11A
  • Ly109
  • ODFR
  • OFE
  • Rank
  • TRANCE-R
  • mRANK
  • CD265
  • FEO
  • LOH18CR1
  • OPTB7
  • OSTS
  • PDB2
  • TRANCER
  • tumor necrosis factor receptor superfamily, member 11a, NFKB activator
  • tumor necrosis factor receptor superfamily, member 11a
  • Tnfrsf11a
  • TNFRSF11A
Epitope
AA 326-616, AA 330-427
19
19
16
13
10
4
4
4
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
103
68
37
Host
Mouse
61
50
9
Clonality
Monoclonal
Conjugate
This TNFRSF11A antibody is un-conjugated
9
6
5
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
72
47
38
26
21
11
8
5
4
4
3
2
1
1
Supplier
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Catalog No. ABIN252472
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.882606 ABIN3042604 IHC (p) WB Rabbit IgG AA 29-44, N-Term Log in to see Polyclonal
2.882606 ABIN252369 WB Rabbit IgG AA 603-613 Log in to see Polyclonal
2.882606 ABIN2470155 IHC WB Rabbit IgG AA 603-613 Log in to see Polyclonal
2.882606 ABIN3222846 IHC WB Rabbit N-Term Log in to see Polyclonal
2.882606 ABIN4349304 CyTOF FACS IP WB Mouse IgG Mix lambda Log in to see 64C1385-1
2.882606 ABIN1860401 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 330-615 Log in to see Polyclonal
2.882606 ABIN4349302 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
2.882606 ABIN2565952 ELISA WB Mouse IgG1 kappa AA 31-130, partial Log in to see 2G2
2.882606 ABIN2435278 ELISA WB Rabbit IgG Log in to see Polyclonal
2.882606 ABIN2500840 ELISA WB HRP Rabbit IgG Log in to see Polyclonal
2.882606 ABIN2500839 ELISA WB FITC Rabbit IgG Log in to see Polyclonal
2.882606 ABIN2500838 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
2.882606 ABIN2500841 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN252036 FACS IHC (p) IHC WB PE Mouse IgG1 kappa AA 326-616, AA 330-427 Log in to see 9A725 10
1 ABIN4900544 ELISA (Capture) FACS WB Mouse IgG1 Extracellular Domain Log in to see 80704 5
1 ABIN372451 IHC (p) WB Rabbit IgG AA 603-613 Log in to see Polyclonal
1 ABIN2470154 FACS IHC WB Mouse IgG1 AA 326-616 Log in to see 9A725
1 ABIN116379 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN116380 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN749258 IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal

Top referenced anti-TNFRSF11A antibody for Western Blotting

Similar anti-TNFRSF11A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies 5 Antibodies
ELISA 5 Antibodies 6 Antibodies
Flow Cytometry (FACS) 17 Antibodies 43 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 18 Antibodies 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 22 Antibodies
Western Blotting (WB) 23 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunoprecipitation (IP) 8 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A) Antibodies
Epitope AA 326-616, AA 330-427
(19), (19), (16), (13), (10), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(103), (68), (37)
Host Mouse
(61), (50), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF11A antibody is un-conjugated
(9), (6), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(72), (47), (38), (26), (21), (11), (8), (5), (4), (4), (3), (2), (1), (1)
Pubmed 14 references available
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Product Details anti-TNFRSF11A Antibody

Target Details TNFRSF11A Application Details Handling References for anti-TNFRSF11A Antibody (ABIN252472) Images
Specificity In RAW cells, a 97 kDa is observed.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Protein G purified
Immunogen A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
Clone 9A725
Isotype IgG1 kappa

Target Details TNFRSF11A

Product Details anti-TNFRSF11A Antibody Application Details Handling References for anti-TNFRSF11A Antibody (ABIN252472) Images back to top
Antigen
Alternative Name RANK/TNFRSF11A (TNFRSF11A Antibody Abstract)
Background Gene Symbol: TNFRSF11A
Gene ID 8792
UniProt Q9Y6Q6
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Handling References for anti-TNFRSF11A Antibody (ABIN252472) Images back to top
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 2-4 μg/mL, Immunohistochemistry 1:20-1:1000, Immunohistochemistry-Paraffin 1:20-1:1000, Flow (Cell Surface), CyTOF-readyBharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry. Use in Flow cell surface reported in scientific literature (PMID 24376119)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details References for anti-TNFRSF11A Antibody (ABIN252472) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TNFRSF11A Antibody (ABIN252472)

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details Handling Images back to top
Product cited in:

Watari, Shibata, Nabeshima, Shinoda, Fukunaga, Kawahara, Karasuyama, Fukushi, Iwamoto, Kuwano, Ono: "Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice." in: Scientific reports, Vol. 6, pp. 19470, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Nanke, Kobashigawa, Yago, Kawamoto, Yamanaka, Kotake: "RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients." in: BioMed research international, Vol. 2016, pp. 4874195, 2016 Method employed by authors: Western Blotting (WB)

Huynh, VonMoss, Smith, Rahman, Felemban, Zuo, Rody, McHugh, Holliday: "Characterization of Regulatory Extracellular Vesicles from Osteoclasts." in: Journal of dental research, Vol. 95, Issue 6, pp. 673-9, 2016 Method employed by authors: Western Blotting (WB)

Kaneko, Ota, Nakashima, Nagasaki, Kodani, Mori, Nagatsu: "Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G₁ phase and protects them from UV light-induced apoptosis." in: Journal of neural transmission (Vienna, Austria : 1996), Vol. 122, Issue 2, pp. 187-99, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Das, Sepahi, Duthie, Clark, Crockett: "RANK receptor oligomerisation in the regulation of NFκB signalling." in: Journal of molecular endocrinology, Vol. 53, Issue 1, pp. 81-91, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Riegel, Maurer, Prior, Stegmaier, Heppert, Wagner, Hänsch: "Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL." in: European journal of immunology, Vol. 42, Issue 4, pp. 975-81, 2012

Izawa, Zou, Chappel, Ashley, Feng, Teitelbaum: "c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton." in: Molecular and cellular biology, Vol. 32, Issue 14, pp. 2943-53, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Cannon, Kraj, Sloan: "Follicle-stimulating hormone promotes RANK expression on human monocytes." in: Cytokine, Vol. 53, Issue 2, pp. 141-4, 2011

Ayon Haro, Ukai, Yokoyama, Kishimoto, Yoshinaga, Hara: "Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation." in: Journal of periodontal research, Vol. 46, Issue 3, pp. 361-73, 2011

Crockett, Mellis, Shennan, Duthie, Greenhorn, Wilkinson, Ralston, Helfrich, Rogers: "Signal peptide mutations in RANK prevent downstream activation of NF-κB." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 26, Issue 8, pp. 1926-38, 2011 Further details: Western Blotting

Huang, Wen, Gao, Zhang, Bai: "An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples." in: Biotechnology letters, Vol. 33, Issue 10, pp. 1939-47, 2011 Further details: Western Blotting

Lee, Kim, Jun, Yoo, Woo, Choi, Lee, Song, Sohn, Park-Min, Ivashkiv, Ji: "Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 10, pp. 5926-34, 2010 (Sample species: Human). Further details: Western Blotting

Bharti, Takada, Shishodia, Aggarwal: "Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6065-76, 2004

Fiumara, Snell, Li, Mukhopadhyay, Younes, Gillenwater, Cabanillas, Aggarwal, Younes: "Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines." in: Blood, Vol. 98, Issue 9, pp. 2784-90, 2001

Images

Product Details anti-TNFRSF11A Antibody Target Details TNFRSF11A Application Details Handling References for anti-TNFRSF11A Antibody (ABIN252472) back to top
Supplier Images
Western Blotting (WB) image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (ABIN252472) Western Blot: RANK Antibody (9A725) [ABIN252472] - Western blot analysis of RANK in 2...
Flow Cytometry (FACS) image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (ABIN252472) Flow Cytometry: RANK Antibody (9A725) [ABIN252472] - Flow analysis of RANK in RAW cel...
 image for anti-TNFRSF11A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator) (AA 326-616) (ABIN252472) Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [ABIN2524728] - Analysis using t...