anti-Fruit Fly (Drosophila melanogaster) GSK3 alpha antibody for Western Blotting

Recommended GSK3 alpha Antibody (supplied by: Log in to see )

Antigen
Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
  • GSK3A
  • CG5182
  • Dmel\\CG5182
  • GSK3a
  • 2700086H06Rik
  • xgsk3
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • gsk3a
  • glycogen synthase kinase 3 alpha
  • CG5182 gene product from transcript CG5182-RA
  • glycogen synthase kinase 3 alpha b
  • GSK3A
  • gsk3a
  • Bm1_31380
  • Pk34A
  • Gsk3a
  • LOC100339192
  • gsk3ab
Epitope
AA 203-219
69
36
25
15
11
9
7
7
6
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Fruit Fly (Drosophila melanogaster), Insect
311
162
134
7
7
6
4
3
3
1
1
1
Host
Mouse
218
118
3
Clonality
Monoclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
24
13
12
7
6
6
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
297
152
138
58
51
48
31
13
7
7
5
4
3
1
Supplier
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Catalog No. ABIN470870
$ 1,013.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN595362 IF IHC WB Rabbit IgG pSer21 Log in to see Polyclonal
1 ABIN211351 WB Mouse IgG1, kappa AA 203-219, Tyr216, pTyr279 Log in to see
1 ABIN601409 IF IHC WB Rabbit IgG Log in to see Polyclonal

General

Antigen Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
Epitope AA 203-219
(69), (36), (25), (15), (11), (9), (7), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Fruit Fly (Drosophila melanogaster), Insect
(311), (162), (134), (7), (7), (6), (4), (3), (3), (1), (1), (1)
Host Mouse
(218), (118), (3)
Clonality
Monoclonal
Conjugate This GSK3 alpha antibody is un-conjugated
(24), (13), (12), (7), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Western Blotting (WB)
(297), (152), (138), (58), (51), (48), (31), (13), (7), (7), (5), (4), (3), (1)
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Product Details anti-GSK3 alpha Antibody

Target Details GSK3 alpha Application Details Handling Images
Specificity Recognizes Glycogen Synthase Kinase 3 (GSK3). Due to extremely high conservation among the known enzymes of many different species, should cross-react with nearly all species homologues. Species cross-reactivity: Mouse, rat, S. pombe, Dictyostelium and human.
Predicted Reactivity Percent identity by BLAST analysis: Drosophila, Mosquito (100%) Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).
Purification Protein G purified
Immunogen Synthetic peptide corresponding to aa203-219 (18aa, C-KQLLHGEPNVSYICSRY) derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK-3/ shaggy enzyme (ovalbumin coupled). Percent identity by BLAST analysis: Drosophila, Mosquito (100%), Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).

Type of Immunogen: Synthetic peptide
Isotype IgG1

Target Details GSK3 alpha

Product Details anti-GSK3 alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name GSK3A / GSK3 Alpha (GSK3a Antibody Abstract)
Background Name/Gene ID: GSK3A
Subfamily: GSK-3
Family: Protein Kinase

Synonyms: GSK3A, GSK-3 alpha, GSK3 alpha
Gene ID 2931
UniProt P49840
Pathways PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling Images back to top
Application Notes Approved: ICC (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunocytochemistry: 10 μg/mL. Demonstrated positive immunostaining for GSK3 in L6 cells fixed with acetone. Positive control: J9000: Jurkat Cell.
Restrictions For Research Use only

Handling

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.