anti-Human GSK3 alpha antibody for Flow Cytometry

Recommended GSK3 alpha Antibody (supplied by: Log in to see )

Antigen
Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
  • GSK3A
  • CG5182
  • Dmel\\CG5182
  • GSK3a
  • 2700086H06Rik
  • xgsk3
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • gsk3a
  • glycogen synthase kinase 3 alpha
  • CG5182 gene product from transcript CG5182-RA
  • glycogen synthase kinase 3 alpha b
  • GSK3A
  • gsk3a
  • Bm1_31380
  • Pk34A
  • Gsk3a
  • LOC100339192
  • gsk3ab
Epitope
pSer9
69
36
25
15
11
9
7
7
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
310
161
133
7
7
6
4
4
3
1
1
1
1
Host
Rabbit
217
119
3
Clonality
Polyclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
24
13
12
7
6
6
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
297
152
138
58
51
48
31
13
7
7
4
3
3
1
Supplier
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Catalog No. ABIN4900620
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.052211 ABIN4900770 CyTOF FACS ICC WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1830875 FACS FITC Mouse IgG1 Log in to see 13A190
1 ABIN5634069 CyTOF FACS ICC WB Rabbit IgG pSer9 Log in to see Polyclonal
1 ABIN5634122 CyTOF FACS ICC WB Rabbit IgG Log in to see Polyclonal

General

Antigen Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
Epitope pSer9
(69), (36), (25), (15), (11), (9), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(310), (161), (133), (7), (7), (6), (4), (4), (3), (1), (1), (1), (1)
Host Rabbit
(217), (119), (3)
Clonality
Polyclonal
Conjugate This GSK3 alpha antibody is un-conjugated
(24), (13), (12), (7), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(297), (152), (138), (58), (51), (48), (31), (13), (7), (7), (4), (3), (3), (1)
Pubmed 3 references available
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Product Details anti-GSK3 alpha Antibody

Target Details GSK3 alpha Application Details Handling References for anti-GSK3 alpha antibody (ABIN4900620) Images
Specificity Detects human, mouse, and rat GSK-3 beta when phosphorylated at S9, and human, mouse, and rat GSK-3 alpha when phosphorylated at S21.
Purification Antigen and protein A Affinity-purified
Sterility 0.2 μm filtered
Immunogen Phosphopeptide containing GSK-3 beta S9 site
Isotype IgG

Target Details GSK3 alpha

Product Details anti-GSK3 alpha Antibody Application Details Handling References for anti-GSK3 alpha antibody (ABIN4900620) Images back to top
Antigen
Alternative Name GSK3A (GSK3a Antibody Abstract)
Background Alternative Names: DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK3 alpha, GSK-3 alpha, GSK3 beta, GSK3A, GSK3B
Gene ID 2931
Pathways PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling References for anti-GSK3 alpha antibody (ABIN4900620) Images back to top
Application Notes Western Blot 0.2 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details References for anti-GSK3 alpha antibody (ABIN4900620) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-GSK3 alpha antibody (ABIN4900620)

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling Images back to top
Product cited in:

Chiu, Liao, Shen, Tang, Jow, Wang, Wu: "HYS-32-Induced Microtubule Catastrophes in Rat Astrocytes Involves the PI3K-GSK3beta Signaling Pathway." in: PLoS ONE, Vol. 10, Issue 5, pp. e0126217, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Kitase, Barragan, Qing, Kondoh, Jiang, Johnson, Bonewald: "Mechanical induction of PGE2 in osteocytes blocks glucocorticoid-induced apoptosis through both the β-catenin and PKA pathways." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 25, Issue 12, pp. 2657-68, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Tang, Arjunan, Lee, Li, Kumar, Hou, Wang, Wardega, Zhang, Dong, Zhang, Zhang, Ding, Fariss, Becker, Lennartsson, Nagai, Cao, Li: "Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation." in: The Journal of experimental medicine, Vol. 207, Issue 4, pp. 867-80, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Images

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling References for anti-GSK3 alpha antibody (ABIN4900620) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer9) antibody (ABIN4900620) Detection of Phospho-GSK-3 alpha/beta (S21/S9) in Jurkat Human Cell Line by Flow Cyto...
Western Blotting (WB) image for anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer9) antibody (ABIN4900620) Detection of Human Phospho-GSK-3 alpha (S21) and GSK-3 beta (S9) by Western Blot. Wes...
Western Blotting (WB) image for anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer9) antibody (ABIN4900620) Detection of Human Phospho-GSK-3 alpha (S21) and GSK-3 beta (S9) by Western Blot. Wes...
Flow Cytometry (FACS) image for anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer9) antibody (ABIN4900620) Detection of Phospho-GSK-3 alpha/beta (S21/S9) in Jurkat Human Cell Line by Flow Cyto...