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anti-Rat (Rattus) GSK3 alpha antibody for Immunocytochemistry

Recommended GSK3 alpha Antibody (supplied by: Log in to see )

Antigen
Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
  • 2700086H06Rik
  • CG5182
  • Dmel\\CG5182
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • GSK3A
  • GSK3a
  • gsk3a
  • xgsk3
Reactivity
Human, Mouse (Murine), Rat (Rattus)
252
120
92
6
2
2
2
2
1
1
Host
Rabbit
206
76
3
Clonality
Polyclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
20
11
10
6
6
6
4
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
229
117
114
37
34
25
11
10
6
3
3
1
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Available images

Catalog No. ABIN257307
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010182734 ABIN1868279 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0010182734 ABIN2972909 ICC IF IP WB Rabbit N-Term Log in to see Polyclonal
0.0010182734 ABIN2987355 ICC IF IHC WB Rabbit IgG pSer21 Log in to see Polyclonal

Similar anti-GSK3 alpha Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 16 Antibodies 33 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Immunochromatography (IC) 2 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 16 Antibodies 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 38 Antibodies 56 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Western Blotting (WB) 75 Antibodies

General

Antigen Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(252), (120), (92), (6), (2), (2), (2), (2), (1), (1)
Host Rabbit
(206), (76), (3)
Clonality
Polyclonal
Conjugate This GSK3 alpha antibody is un-conjugated
(20), (11), (10), (6), (6), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(229), (117), (114), (37), (34), (25), (11), (10), (6), (3), (3), (1)
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Product Details anti-GSK3 alpha Antibody

Target Details GSK3 alpha Application Details Handling Images
Specificity GSK3 alpha (specific to a.a. 21) detects endogenous levels of total GSK3 alpha protein. Does not detect GSK-3b protein
Purification affinity purified
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human GSK3 alpha around a.a 21 (T-S-S-F-A)
Isotype IgG

Target Details GSK3 alpha

Product Details anti-GSK3 alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name GSK-3 alpha (GSK3a Antibody Abstract)
Background Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases,Cdc2/cdkx subfamily, GSK3 subsubfamily. It is implicated in the hormonal control ofseveral regulatory proteins including glycogen synthase, myb, and the transcriptionfactor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulinstimulates the dephosphorylation of glycogen synthase at the sites phosphorylated byGSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogensynthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta.The two isoforms share 97% sequence similarity within their catalytic domains. GSK3has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation,cell differentiation, microtubule dynamics and cell motility.
Gene Symbol: GSK3A
Gene ID 2931, 50686
Research Area Cardiovascular, Hypertrophy, Atherosclerosis, Cancer, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling Images back to top
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100~1:200, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Western Blot 1:100-1:2000
Restrictions For Research Use only

Handling

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, and 50 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.

Images

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-GSK3 alpha antibody (Glycogen Synthase Kinase 3 alpha) (ABIN257307) Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue usin...
Western Blotting (WB) image for anti-GSK3 alpha antibody (Glycogen Synthase Kinase 3 alpha) (ABIN257307) Western blot analysis of extracts from ovary cancer cells using GSK3 alpha (Ab-21) an...