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anti-Cow (Bovine) PDK4 antibody for Immunocytochemistry

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
AA 100-150
23
22
13
12
10
6
5
5
5
5
5
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
117
48
12
1
1
1
Host
Rabbit
120
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
9
8
7
7
7
6
2
2
2
Application
Immunocytochemistry (ICC), Western Blotting (WB)
119
78
55
14
10
4
2
2
1
Supplier
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Available images

Catalog No. ABIN446475
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Similar anti-PDK4 Antibodies

Application / Reactivity Cow (Bovine)
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Western Blotting (WB) 5 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope AA 100-150
(23), (22), (13), (12), (10), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(117), (48), (12), (1), (1), (1)
Host Rabbit
(120), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(9), (8), (7), (7), (7), (6), (2), (2), (2)
Application Immunocytochemistry (ICC), Western Blotting (WB)
(119), (78), (55), (14), (10), (4), (2), (2), (1)
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling Images
Cross-Reactivity (Details) Predicted to react with bovine based on 100% sequence homology.
Purification affinity purified
Immunogen Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 100-150). Immunogen sequence has 94% homology to rat

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background Gene Symbol: PDK4
Gene ID 5166, 27273, 89813
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling Images back to top
Application Notes This PDK4 antibody is useful for Western blot, where a band is seen at ~49 kDa. This product is not recommended for ICC, as nonspecific nuclear staining was observed in HeLa cells.
Protocol Protocol specific for PDK4 Antibody Procedure Guide for NBP1-07047 - PDK4 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-PDK4 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Images back to top
Format Liquid
Concentration 1.27 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (AA 100-150) (ABIN446475) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 100-150) antibody