anti-Cow (Bovine) PDK4 antibody for Western Blotting

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pdk4
  • LOC100230570
  • Pdk4
  • PDK4
Epitope
N-Term
29
19
17
17
17
12
10
10
8
6
6
6
6
5
5
4
3
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
149
71
15
1
1
1
1
1
Host
Rabbit
151
20
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
10
8
7
7
7
4
4
4
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
151
78
59
35
25
18
4
3
2
1
Options
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Catalog No. ABIN2777592
$ 289.00
Plus shipping costs $45.00

Top referenced anti-PDK4 antibody for Western Blotting

Similar anti-PDK4 Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Dog (Canine) Pig (Porcine) Rat (Rattus) Horse (Equine) Rabbit Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies
Western Blotting (WB) 130 Antibodies 64 Antibodies 1 Antibodies 1 Antibodies 2 Antibodies 13 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 6 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 1 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope N-Term
(29), (19), (17), (17), (17), (12), (10), (10), (8), (6), (6), (6), (6), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(149), (71), (15), (1), (1), (1), (1), (1)
Host Rabbit
(151), (20)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(11), (10), (8), (7), (7), (7), (4), (4), (4), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(151), (78), (59), (35), (25), (18), (4), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling ProductDetails: References for anti-PDK4 antibody (ABIN2777592) Images
Blocking Peptide Blocking peptide for this product available: ABIN981542
Sequence MKAARFVLRS AGSLNGAGLV PREVEHFSRY SPSPLSMKQL LDFGSENACE
Predicted Reactivity Cow: 92%, Dog: 92%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against PDK4. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PDK4

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling ProductDetails: References for anti-PDK4 antibody (ABIN2777592) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Alias Symbols: FLJ40832
Protein Interaction Partner: UBC, DLAT, PDK4, PDHA1, TRIM63,
Protein Size: 411
Molecular Weight 46 kDa
Gene ID 5166
NCBI Accession NM_002612, NP_002603
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling ProductDetails: References for anti-PDK4 antibody (ABIN2777592) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 411 AA

Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details ProductDetails: References for anti-PDK4 antibody (ABIN2777592) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PDK4 antibody (ABIN2777592)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Product cited in:

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006

Background publications

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling ProductDetails: References for anti-PDK4 antibody (ABIN2777592) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) Sample Type: Huh7 HepG2 (50ug)
Primary Antibody Dilution: 1:500
...
Immunohistochemistry (IHC) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody (Image 3)
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) WB Suggested Anti-PDK4
Antibody Titration: 0.2-1 µg/mL
Positive Control...