You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PDK4 antibody for Immunocytochemistry

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
AA 100-150, Internal Region
25
24
23
16
13
13
12
10
5
5
5
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
139
60
12
1
1
1
Host
Rabbit
142
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
9
7
7
7
7
4
4
3
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
140
78
57
17
16
8
4
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN446469
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009993567 ABIN446476 ICC IF WB Rabbit AA 200-250, Internal Region Log in to see Polyclonal
0.0009993567 ABIN1860174 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 73-316 Log in to see Polyclonal
0.0009993567 ABIN4344609 ICC IF WB Alexa Fluor 647 Rabbit AA 200-250, Internal Region Log in to see Polyclonal
0.0009993567 ABIN4344603 ICC IF WB DyLight 405 Rabbit AA 200-250, Internal Region Log in to see Polyclonal
0.0009993567 ABIN4344605 ICC IF WB Alexa Fluor 405 Rabbit AA 200-250, Internal Region Log in to see Polyclonal
0.0009993567 ABIN4344607 ICC IF WB Alexa Fluor 488 Rabbit AA 200-250, Internal Region Log in to see Polyclonal
0.0009993567 ABIN4344611 ICC IF WB Alexa Fluor 700 Rabbit AA 200-250, Internal Region Log in to see Polyclonal
0.0009993567 ABIN4344604 ICC IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PDK4 antibody for Immunocytochemistry

Similar anti-PDK4 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 27 Antibodies 61 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 9 Antibodies
Immunofluorescence (IF) 6 Antibodies 18 Antibodies
Immunohistochemistry (IHC) 15 Antibodies 57 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope AA 100-150, Internal Region
(25), (24), (23), (16), (13), (13), (12), (10), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(139), (60), (12), (1), (1), (1)
Host Rabbit
(142), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(11), (9), (7), (7), (7), (7), (4), (4), (3), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(140), (78), (57), (17), (16), (8), (4), (2), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN446469) Images
Cross-Reactivity (Details) Predicted to react with bovine based on 100% sequence homology.
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 100-150). [Swiss-Prot# Q16654]

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling References for anti-PDK4 Antibody (ABIN446469) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background Gene Symbol: PDK4
Molecular Weight Theoretical MW: 49 kDa
Gene ID 5166, 27273, 89813
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling References for anti-PDK4 Antibody (ABIN446469) Images back to top
Application Notes Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PDK4 Antibody Procedure Guide for NBP1-07047 - PDK4 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-PDK4 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details References for anti-PDK4 Antibody (ABIN446469) Images back to top
Format Liquid
Concentration 1.35 mg/mL
Buffer PBS, 30 % glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-PDK4 Antibody (ABIN446469)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Product cited in:

Golias, Papandreou, Sun, Kumar, Brown, Swanson, Pai, Jaitin, Le, Teknos, Denko: "Hypoxic repression of pyruvate dehydrogenase activity is necessary for metabolic reprogramming and growth of model tumours." in: Scientific reports, Vol. 6, pp. 31146, 2016 (Sample species: Human). Further details: Western Blotting

Ho, Coomber: "Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells." in: Experimental cell research, Vol. 331, Issue 1, pp. 73-81, 2015 (Sample species: Human).

Hou, Zhang, Han, Ge, Ma, Zhang, Moley, Schedl, Wang: "Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation." in: Journal of cell science, Vol. 128, Issue 13, pp. 2319-29, 2015 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Oikawa, Mano, Takahashi, Kakinuma: "Remote ischemic preconditioning with a specialized protocol activates the non-neuronal cardiac cholinergic system and increases ATP content in the heart." in: International immunopharmacology, Vol. 29, Issue 1, pp. 181-4, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Kainulainen, Papaioannou, Silvennoinen, Autio, Saarela, Oliveira, Nyqvist, Pasternack, t Hoen, Kujala, Ritvos, Hulmi: "Myostatin/activin blocking combined with exercise reconditions skeletal muscle expression profile of mdx mice." in: Molecular and cellular endocrinology, Vol. 399, pp. 131-42, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rinnankoski-Tuikka, Silvennoinen, Torvinen, Hulmi, Lehti, Kivelä, Reunanen, Kainulainen: "Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle." in: Nutrition & metabolism, Vol. 9, Issue 1, pp. 53, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Connaughton, Chowdhury, Attia, Song, Zhang, Elam, Cook, Park: "Regulation of pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression by glucocorticoids and insulin." in: Molecular and cellular endocrinology, Vol. 315, Issue 1-2, pp. 159-67, 2010

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN446469) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (AA 100-150) (ABIN446469) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 100-150), (Internal Region) antibody