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anti-Human PDK4 antibody for Immunocytochemistry

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
AA 200-250, Internal Region
23
22
13
12
10
6
5
5
5
5
5
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Human
117
48
12
1
1
1
Host
Rabbit
120
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
9
8
7
7
7
6
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
119
78
55
14
10
4
2
2
1
Supplier
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Available images

Catalog No. ABIN446482
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010025558 ABIN1860174 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 73-316 Log in to see Polyclonal

Similar anti-PDK4 Antibodies

Application / Reactivity Human
ELISA 61 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Western Blotting (WB) 103 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope AA 200-250, Internal Region
(23), (22), (13), (12), (10), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (48), (12), (1), (1), (1)
Host Rabbit
(120), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(9), (8), (7), (7), (7), (6), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(119), (78), (55), (14), (10), (4), (2), (2), (1)
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling Images
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 200-250). [Swiss-Prot# Q16654]

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background Gene Symbol: PDK4
Molecular Weight Theoretical MW: 49 kDa
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling Images back to top
Application Notes Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PDK4 Antibody Procedure Guide for NBP1-07049 - PDK4 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-PDK4 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Images back to top
Format Liquid
Concentration 1.24 mg/mL
Buffer PBS, 30 % glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (AA 200-250) (ABIN446482) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 200-250), (Internal Region) antibody