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anti-Mouse (Murine) PDK4 antibody for Enzyme Immunoassay

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
C-Term
26
24
22
17
13
13
12
10
5
5
5
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
139
60
12
1
1
1
Host
Rabbit
142
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
9
7
7
7
7
4
4
3
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
140
78
57
17
17
9
3
2
1
Supplier
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Catalog No. ABIN359098
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN615174 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PDK4 antibody for Enzyme Immunoassay

Similar anti-PDK4 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 27 Antibodies 61 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 9 Antibodies
Immunofluorescence (IF) 6 Antibodies 18 Antibodies
Immunohistochemistry (IHC) 15 Antibodies 57 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope C-Term
(26), (24), (22), (17), (13), (13), (12), (10), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(139), (60), (12), (1), (1), (1)
Host Rabbit
(142), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(11), (9), (7), (7), (7), (7), (4), (4), (3), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(140), (78), (57), (17), (17), (9), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN359098) Images
Specificity This antibody reacts to PDK4.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PDK4.
Isotype Ig

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling References for anti-PDK4 Antibody (ABIN359098) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase lipoamide kinase isozyme 4 mitochondrial
Gene ID 5166, 9606
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling References for anti-PDK4 Antibody (ABIN359098) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details References for anti-PDK4 Antibody (ABIN359098) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDK4 Antibody (ABIN359098)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Background publications

Hsieh, Das, Sambandam, Zhang, Nahlé: "Regulation of the PDK4 isozyme by the Rb-E2F1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 41, pp. 27410-7, 2008

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN359098) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (C-Term) (ABIN359098) (LEFT) The PDK4 Antibody (C-term) is used in Western blot to detect PDK4 in mouse ske...