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|Application / Reactivity||Human|
|Dot Blot (DB)||120 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies|
|Immunofluorescence (IF)||46 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||104 Antibodies|
|Immunohistochemistry (IHC)||137 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||64 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||202 Antibodies|
|Antigen||Tuberous Sclerosis 2 (TSC2) Antibodies|
|Conjugate||This TSC2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-TSC2 AntibodyTarget Details TSC2 Application Details Handling References for anti-TSC2 Antibody (ABIN358411) Images
|Specificity||This antibody detects TSC2 pSer1420.|
|Purification||Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1420 of human TSC2.|
Target Details TSC2Product Details anti-TSC2 Antibody Application Details Handling References for anti-TSC2 Antibody (ABIN358411) Images back to top
|Alternative Name||Tuberin / TSC2 (TSC2 Antibody Abstract)|
|Background||Mutations in TSC2 lead to tuberous sclerosis complex. The protein is believed to be a tumor suppressor and is able to specifically stimulate the intrinsic GTPase activity of the Ras-related protein RAP1A and RAB5. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking.Synonyms: TSC4, Tuberous sclerosis 2 protein|
|Molecular Weight||200749 Da|
|Gene ID||7249, 9606|
|Pathways||RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-TSC2 Antibody Target Details TSC2 Handling References for anti-TSC2 Antibody (ABIN358411) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TSC2 Antibody Target Details TSC2 Application Details References for anti-TSC2 Antibody (ABIN358411) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-TSC2 Antibody (ABIN358411)Product Details anti-TSC2 Antibody Target Details TSC2 Application Details Handling Images back to top
Li, Inoki, Guan: "Biochemical and functional characterizations of small GTPase Rheb and TSC2 GAP activity." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 7965-75, 2004 (PubMed).
Corradetti, Inoki, Bardeesy et al.: "Regulation of the TSC pathway by LKB1: evidence of a molecular link between tuberous sclerosis complex and Peutz-Jeghers syndrome." in: Genes & development, Vol. 18, Issue 13, pp. 1533-8, 2004 (PubMed).
Karbowniczek, Cash, Cheung et al.: "Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 29930-7, 2004 (PubMed).
Birchenall-Roberts, Fu, Bang et al.: "Tuberous sclerosis complex 2 gene product interacts with human SMAD proteins. A molecular link of two tumor suppressor pathways." in: The Journal of biological chemistry, Vol. 279, Issue 24, pp. 25605-13, 2004 (PubMed).
Lewis, Thomas, Murphy et al.: "Genotype and psychological phenotype in tuberous sclerosis." in: Journal of medical genetics, Vol. 41, Issue 3, pp. 203-7, 2004 (PubMed).
ImagesProduct Details anti-TSC2 Antibody Target Details TSC2 Application Details Handling References for anti-TSC2 Antibody (ABIN358411) back to top