anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) Antibodies

CIITA encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. Additionally we are shipping Class II, Major Histocompatibility Complex, Transactivator Proteins (3) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CIITA 12265 P79621
CIITA 4261 P33076
CIITA 85483  
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Top anti-Class II, Major Histocompatibility Complex, Transactivator Antibodies at antibodies-online.com

Showing 10 out of 49 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-CIITA Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:62500  Positive Control:  Human Liver 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of extracts from K562 cells, using CIITA Antibody. The lane on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated IHC (p), WB   100 μg Log in to see 4 to 6 Days
$280.00
Details
Dog Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
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Cow Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
Details
Human Rabbit Un-conjugated ELISA, IHC, IHC (p), WB Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 7 to 9 Days
$484.00
Details
Human Rabbit Un-conjugated ELISA, WB 100 μL Log in to see 7 to 9 Days
$639.83
Details
Human Rabbit Un-conjugated IP   0.1 mL Log in to see 6 to 8 Days
$533.50
Details
Human Rabbit Un-conjugated WB <b>Western blot</b> (WB) analysis of CIITA antibody in extracts from K562 cells. 0.1 mg Log in to see 6 to 8 Days
$390.50
Details
Pig Rabbit Un-conjugated WB Western blot of Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced DYKDDDDK-CIITA aa 1-333, was tested by western blot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-DYKDDDDK-CIITA-336-884 and pcDNA3-HA-CIITA. For transfection, Fugene 6 (Roche) was used according to the manufacturer's instructions for a 6-well plate format. Cells were lysed 24 h post-transfection in 200 uL of 1x SDS-sample buffer, heated at 96C for 5', and vortexed for 30 sec. Samples (10 uL each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45' using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel A, both samples on PVDF were incubated with 10 ug/mL mouse anti-DYKDDDDK antibody (Sigma) for 45'. After 5X washes with TTBS, reaction with ALP rabbit anti-mouse IgG at 200 ng/mL proceeded for 45' following again by washing as before. The blot was developed using BCIP/NBT. This blot demonstrates that DYKDDDDK-CIITA-336-842 was successfully over-expressed in the Cos-7 cells. In panel B, both samples on PVDF were incubated with a 1:500 dilution of anti-CIITA (1-333) for 45'. After 5X washes with TTBS, reaction with HRP goat anti-rabbit IgG at 10 ng/mL proceeded for 45' following again by washing as before. The membrane was covered with Pico West Substrate solution (Pierce) for 5' and was then placed between the two layers of a standard sheet protector. Kodak O-MAT film was exposed to the blot for 30 sec and was immediately developed. The lane containing the lysate of pcDNA3-HA-CIITA transfected cells contains a single band at ~130 kD molecular weight, whereas the lane containing lysate from pcDNA3-DYKDDDDK-CIITA-336-842 transfected cells shows no reactivity. This blot demonstrates that anti-CIITA (1-333) is specific for amino acids 1-333 of CIITA and that the antibody is not cross reactive with the DYKDDDDK portion of the immunogen. Western blot of Anti-CIITA  antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by western blot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA. For transfection, Fugene 6 (Roche) was used according to the manufacturer's instructions for a 6-well plate format. Cells were lysed 24 h post-transfection in 200 uL of 1x SDS-sample buffer, heated at 96C for 5', and vortexed for 30 sec. Samples (10 uL each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45' using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel A, both samples on PVDF were incubated with 10 ug/mL mouse anti-FLAG antibody (Sigma) for 45'. After 5X washes with TTBS, reaction with ALP rabbit anti-mouse IgG at 200 ng/mL proceeded for 45' following again by washing as before. The blot was developed using BCIP/NBT. This blot demonstrates that FLAG-CIITA-336-842 was successfully over-expressed in the Cos-7 cells. In panel B, both samples on PVDF were incubated with a 1:500 dilution of  anti-CIITA  for 45'. After 5X washes with TTBS, reaction with HRP goat anti-rabbit IgG at 10 ng/mL proceeded for 45' following again by washing as before. The membrane was covered with Pico West Substrate solution (Pierce) for 5' and was then placed between the two layers of a standard sheet protector. Kodak O-MAT film was exposed to the blot for 30 sec and was immediately developed. The lane containing the lysate of pcDNA3-HA-CIITA transfected cells contains a single band at ~130 kD molecular weight, whereas the lane containing lysate from pcDNA3-FLAG-CIITA-336-842 transfected cells shows no reactivity. This blot demonstrates that anti-CIITA  is specific for amino acids 1-333 of CIITA and that the antibody is not cross reactive with the FLAG portion of the immunogen. 100 μL Log in to see 1 to 2 Days
$389.00
Details

CIITA Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality
Mouse (Murine)


Human , , ,
,
,
Rat (Rattus)


Top referenced anti-Class II, Major Histocompatibility Complex, Transactivator Antibodies

  1. Pig (Porcine) Polyclonal CIITA Primary Antibody for WB - ABIN97339 : Koues, Dudley, Mehta, Greer: The 19S proteasome positively regulates histone methylation at cytokine inducible genes. in Biochimica et biophysica acta 2009 (PubMed)

More Antibodies against Class II, Major Histocompatibility Complex, Transactivator Interaction Partners

Mouse (Murine) Class II, Major Histocompatibility Complex, Transactivator (CIITA) interaction partners

Human Class II, Major Histocompatibility Complex, Transactivator (CIITA) interaction partners

Class II, Major Histocompatibility Complex, Transactivator (CIITA) Antigen Profile

Protein Summary

This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the 'master control factor' for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.

Gene names and symbols associated with CIITA

  • class II, major histocompatibility complex, transactivator (CIITA) antibody
  • class II major histocompatibility complex transactivator (CIITA) antibody
  • MHC class II transactivator (LOC710761) antibody
  • class II, major histocompatibility complex, transactivator (ciita) antibody
  • MHC class II transactivator (LOC100443575) antibody
  • class II transactivator (Ciita) antibody
  • MHC class II transactivator (LOC100736732) antibody
  • class II, major histocompatibility complex, transactivator (Ciita) antibody
  • C2TA antibody
  • CIITA antibody
  • CIITAIV antibody
  • EG669998 antibody
  • Gm9475 antibody
  • Mhc2ta antibody
  • NLRA antibody

Protein level used designations for CIITA

class II, major histocompatibility complex, transactivator , class II transactivator-like , class II transactivator , MHC class II transactivator-like , MHC class II transactivator , MHC class II transactivator type III , NLR family, acid domain containing , nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing , MHC class II transactivator type IV

GENE ID SPECIES
427676 Gallus gallus
453914 Pan troglodytes
508720 Bos taurus
710761 Macaca mulatta
100003537 Danio rerio
100056546 Equus caballus
100338894 Oryctolagus cuniculus
100402996 Callithrix jacchus
100443575 Pongo abelii
12265 Mus musculus
100736732 Sus scrofa
4261 Homo sapiens
85483 Rattus norvegicus
490008 Canis lupus familiaris
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