anti-Guinea Pig ATG5 antibody for Immunohistochemistry

Recommended ATG5 Antibody (supplied by: Log in to see )

Antigen
ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) Antibodies
  • APG5
  • APG5-LIKE
  • APG5L
  • ASP
  • hAPG5
  • ATG5
  • CG1643
  • DmAtg5
  • Dmel\\CG1643
  • atg5
  • 2010107M05Rik
  • 3110067M24Rik
  • AW319544
  • Apg5l
  • Atg5l
  • C88337
  • Paddy
  • apg5l
  • zgc:100934
  • ATATG5
  • AUTOPHAGY 5
  • MKP11.20
  • MKP11_20
  • autophagy related 5
  • Autophagy-related 5
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • autophagy related 5 L homeolog
  • similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12
  • autophagy protein Apg5 family
  • ATG5
  • Atg5
  • atg5
  • atg5.L
  • APG5
Epitope
C-Term
75
28
22
15
12
11
8
7
7
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
338
127
98
31
27
23
18
10
9
5
4
3
3
2
2
2
2
1
1
1
1
Host
Rabbit
264
61
13
11
2
Clonality
Polyclonal
Conjugate
This ATG5 antibody is un-conjugated
15
15
15
12
12
12
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
284
140
139
73
52
42
39
13
12
8
6
5
3
2
1
1
1
Options
Supplier
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Catalog No. ABIN2785620
$ 319.00
Plus shipping costs $45.00

General

Antigen ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) Antibodies
Epitope C-Term
(75), (28), (22), (15), (12), (11), (8), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(338), (127), (98), (31), (27), (23), (18), (10), (9), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(264), (61), (13), (11), (2)
Clonality
Polyclonal
Conjugate This ATG5 antibody is un-conjugated
(15), (15), (15), (12), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(284), (140), (139), (73), (52), (42), (39), (13), (12), (8), (6), (5), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ATG5 Antibody

Target Details ATG5 Application Details Handling References for anti-ATG5 antibody (ABIN2785620) Images
Blocking Peptide Blocking peptide for this product available: ABIN973582
Sequence DPEDGEKKNQ VMIHGIEPML ETPLQWLSEH LSYPDNFLHI SIIPQPTD
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against ATG5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human ATG5
Plasmids, Primers & others

Target Details ATG5

Product Details anti-ATG5 Antibody Application Details Handling References for anti-ATG5 antibody (ABIN2785620) Images back to top
Antigen
Alternative Name ATG5 (ATG5 Antibody Abstract)
Background ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Alias Symbols: APG5, APG5L, ASP, hAPG5, APG5-LIKE
Protein Interaction Partner: TEKT4, CCHCR1, WIPI2, KEAP1, VCP, MEOX2, FADD, ATG16L1, RB1CC1, EXOC4, ATG3, ATG12, CAV1, HTT, WDFY3, TAB3, TAB2, TECPR1, ELAVL1, UBC, SIRT1, TKT, MAP1LC3C, MAP1LC3B, ATG10, ATG5, IMPDH2,
Protein Size: 275
Molecular Weight 32 kDa
Gene ID 9474
NCBI Accession NM_004849, NP_004840
UniProt Q9H1Y0
Pathways Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy

Application Details

Product Details anti-ATG5 Antibody Target Details ATG5 Handling References for anti-ATG5 antibody (ABIN2785620) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 275 AA

Restrictions For Research Use only

Handling

Product Details anti-ATG5 Antibody Target Details ATG5 Application Details References for anti-ATG5 antibody (ABIN2785620) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATG5 antibody (ABIN2785620)

Product Details anti-ATG5 Antibody Target Details ATG5 Application Details Handling Images back to top
Product cited in:

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007

Images

Product Details anti-ATG5 Antibody Target Details ATG5 Application Details Handling References for anti-ATG5 antibody (ABIN2785620) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (C-Term) antibody (ABIN2785620) anti-ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (C-Term) antibody
Immunohistochemistry (IHC) image for anti-ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (C-Term) antibody (ABIN2785620) anti-ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (C-Term) antibody (Image 2)