Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody

Details for Product No. ABIN263212
Target
Binding Specificity
Internal Region
10
4
3
2
2
1
1
1
1
Reactivity
Human
38
7
6
3
3
3
3
3
3
3
2
2
2
2
1
1
1
Host
Goat
25
8
6
Clonality
Polyclonal
Conjugate
Un-conjugated
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
26
17
11
10
8
3
1
1
1
Options
Purpose TXNDC4
Immunogen C-EQKDSDNYRVFER
Blocking Peptide Blocking peptide for this product available: ABIN369045
Sequence EQKDSDNYRV FER
Isotype IgG
Predicted Reactivity Human, Mouse, Rat, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name TXNDC4 (ERP44 Antibody Abstract)
Background TXNDC4 , thioredoxin domain containing 4 (endoplasmic reticulum) , ERP44, KIAA0573, OTTHUMP00000063799, endoplasmic reticulum resident protein 44 kDa
Gene ID 23071
NCBI Accession NP_055866
Pathways Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome
Application Notes

DS WB Results: Approx 47 kDa band observed in lysates of cell line K562 (calculated MW of 47.0 kDa according to NP_055866.1). Recommended concentration: 0.2-0.6 μg/mL. An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody (ABIN263212) ABIN263212 (0.2µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary ...