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DS WB Results: Approx 47 kDa band observed in lysates of cell line K562 (calculated MW of 47.0 kDa according to NP_055866.1). Recommended concentration: 0.2-0.6 μg/mL. An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Peptide ELISA: antibody detection limit dilution 1:32000.