FGF2 antibody

Catalog No. ABIN1101522

Product Details anti-FGF2 Antibody

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FGF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FGF-basic

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human FGF-basic.

Type of Immunogen: Cell extract

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (0.25 - 0.5 μg/mL)

Usage: Immunohistochemistry: LS-B7410 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 μg/mL is recommended. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human FGF-basic when used with biotinylated antigen affinity purified anti-human FGF-basic (LS-C104313) as the detection antibody at a concentration of approximately 0.125-0.25 μg/mL. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-basic is 1-2 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF2 / BFGF (FGF2 Products)

Synonyms: BFGF antibody, FGF-2 antibody, FGFB antibody, HBGF-2 antibody, Fgf-2 antibody, Fgfb antibody, bFGF antibody, fibroblast growth factor 2 antibody, fibroblast growth factor 2 (basic) antibody, FGF2 antibody, Fgf2 antibody, fgf2 antibody

Background: Name/Gene ID: FGF2
Family: HBGF

Synonyms: FGF2, Basic fibroblast growth factor, FGFB, Fibroblast growth factor 2, FGF-2, Prostatropin, BFGF, HBGF-2

Gene ID: 2247

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins