FGF4 antibody

Catalog No. ABIN1101526

Product Details anti-FGF4 Antibody

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FGF-4

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human FGF-4.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7422 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hFGF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-4 (LS-C104316) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-4. Western Blot: To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FGF4

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Alternative Name: FGF4 (FGF4 Products)

Synonyms: HBGF-4 antibody, HST antibody, HST-1 antibody, HSTF1 antibody, K-FGF antibody, KFGF antibody, Fgf-4 antibody, Fgfk antibody, Hst1 antibody, Hstf-1 antibody, KS3 antibody, hst antibody, hst-1 antibody, kFGF antibody, Hst antibody, fibroblast growth factor 4 antibody, FGF4 antibody, Fgf4 antibody

Background: Name/Gene ID: FGF4
Family: HBGF

Synonyms: FGF4, Fibroblast growth factor 4, FGF-4, Hst, HSTF-1, Kaposi sarcoma oncogene, K-FGF, KFGF, HBGF-4, HST-1, Oncogene HST, HSTF1, KS3, Transforming protein KS3

Gene ID: 2249

UniProt: P08620

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance