IL-6 antibody (HRP)
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- Target See all IL-6 (IL6) Antibodies
- IL-6 (IL6) (Interleukin 6 (IL6))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL-6 antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- This purified antibody has been heated to 56 °C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
- Purification
- Purified, heated to 56°C for 30 minutes
- Immunogen
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This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E. coli.
Type of Immunogen: Recombinant protein - Isotype
- IgG
- Top Product
- Discover our top product IL6 Primary Antibody
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- Application Notes
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Approved: ELISA, IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)
Usage: This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kD in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- IL-6 (IL6) (Interleukin 6 (IL6))
- Alternative Name
- IL6 (IL6 Products)
- Synonyms
- BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody
- Background
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Name/Gene ID: IL6
Family: Hormone
Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6 - Gene ID
- 3569
- UniProt
- P05231
- Pathways
- TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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