NOTCH4 antibody (Extracellular Domain)

Catalog No. ABIN1103305

Product Details anti-NOTCH4 Antibody

Target: NOTCH4 (Notch 4 (NOTCH4))

Binding Specificity: AA 1261-1273, Extracellular Domain

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: ELISA

Specificity: Human NOTCH4. The immunizong peptide represents part of the extracellular domain.

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Bovine, Dog, Panda, Horse, Porcine (93%) Hamster, Opossum (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DHFHNGHCEKGCNN from an internal region of human NOTCH4 (NP_004548.3). Percent identity by BLAST analysis: Mouse, Rat (100%), Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Bovine, Dog, Panda, Horse, Porcine (93%), Hamster, Opossum (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 27kD band in all human, mouse and rat tissue and cell line lysates tested after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kD according to NP_004548.3. The 27kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: NOTCH4 (NOTCH4 Products)

Synonyms: INT3 antibody, NOTCH3 antibody, Int-3 antibody, Int3 antibody, N4 antibody, Notch4 antibody, notch 4 antibody, neurogenic locus notch homolog protein 4 antibody, NOTCH4 antibody, Notch4 antibody, LOC100712850 antibody

Background: Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

Gene ID: 4855

NCBI Accession: NP_004548

UniProt: Q99466

Pathways: Notch Signaling