POLB antibody (C-Term)

Catalog No. ABIN1103699

Product Details anti-POLB Antibody

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody reacts with overexpressed and endogenous POLbeta protein. A BLAST analysis was used to suggest reactivity with POLbeta from human, bovine, and Xenopus laevis based on a 100 % homology with the immunizing sequence. Cross-reactivity with POLbeta from other sources has not been determined.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POL protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (0.5 μg/mL)

Usage: This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kD in size corresponding to POLbeta by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB / DNA Polymerase Beta (POLB Products)

Synonyms: wu:fa97a05 antibody, zgc:109924 antibody, POLB antibody, DDBDRAFT_0186152 antibody, DDBDRAFT_0215784 antibody, DDBDRAFT_0232376 antibody, DDB_0186152 antibody, DDB_0215784 antibody, DDB_0232376 antibody, A430088C08Rik antibody, polymerase (DNA directed), beta antibody, DNA polymerase beta antibody, DNA polymerase X family protein antibody, polymerase (DNA directed), beta S homeolog antibody, polb antibody, POLB antibody, polB antibody, polb.S antibody, Polb antibody

Background: Name/Gene ID: POLB

Synonyms: POLB, DNA pol beta, Mutant DNA polymerase beta, DNA polymerase beta, DNA polymerase beta subunit

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair