RPS6KA1 antibody (pSer732)

Catalog No. ABIN1104063

Product Details anti-RPS6KA1 Antibody

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Binding Specificity: pSer732

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100 % homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on >90 % homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1 μg/mL)

Usage: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kD in size corresponding to p90 RSK1 pS732 protein is expected in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Alternative Name: RPS6KA1 / RSK1 (RPS6KA1 Products)

Synonyms: HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Rsk1 antibody, p90rsk antibody, rsk antibody, RPS6KA antibody, hu-1 antibody, mapkapk1a antibody, rsk1 antibody, wu:fc56h07 antibody, zgc:152654 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase polypeptide 1 antibody, ribosomal protein S6 kinase A1-like antibody, ribosomal protein S6 kinase A1 L homeolog antibody, ribosomal protein S6 kinase a, polypeptide 1 antibody, RPS6KA1 antibody, Rps6ka1 antibody, RPS6KA1L antibody, rps6ka1.L antibody, rps6ka1 antibody

Background: Name/Gene ID: RPS6KA1
Subfamily: RSK
Family: Protein Kinase

Synonyms: RPS6KA1, MAPKAPK-1a, p90RSK, p90-RSK 1, Pp90RSK1, Ribosomal S6 kinase 1, RSK-1, S6K-alpha 1, MAPKAP kinase 1a, MAPKAPK1A, p90 s6 kinase, p90S6K, RSK, RSK1, HU-1, p90RSK1, S6K-alpha-1

Gene ID: 6195

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades