MUC1 antibody

Catalog No. ABIN1106101

Product Details anti-MUC1 Antibody

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: VU-12E1 reacts with MUC1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: A Balb/c mouse was immunized with live cells (10e7 cells/dose in PBS) of human breast cancer cell line ZR-75-1. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.

Clone: VU-12E1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: PBS, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: CD227 / Mucin-1 / MUC1 (MUC1 Products)

Synonyms: CA 15-3 antibody, CD227 antibody, EMA antibody, H23AG antibody, KL-6 antibody, MAM6 antibody, MCKD1 antibody, MUC-1 antibody, MUC-1/SEC antibody, MUC-1/X antibody, MUC1/ZD antibody, PEM antibody, PEMT antibody, PUM antibody, Muc-1 antibody, mucin 1, cell surface associated antibody, mucin 1, transmembrane antibody, MUC1 antibody, Muc1 antibody

Background: MUC1 is a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of mAb VU12E1 is the 5-mer PDTRP of the MUC1 tandem repeat as established with Synonyms: Breast carcinoma-associated antigen DF3, CA 15-3, Carcinoma-associated mucin, DF3, EMA, Episialin, H23AG, MUC-1, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

Gene ID: 4582

NCBI Accession: NP_001018016

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling