ITGA4 antibody (Biotin)
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- Target See all ITGA4 Antibodies
- ITGA4 (Integrin alpha 4 (ITGA4))
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Reactivity
- Human
- Host
- Please inquire
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Clonality
- Monoclonal
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Conjugate
- This ITGA4 antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Characteristics
- The CD49d antigen is primarily expressed on T and B lymphocytes and weakly expressed on monocytes.
- Clone
- ALC1-1
- Isotype
- IgG1
- Top Product
- Discover our top product ITGA4 Primary Antibody
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- Application Notes
- It is recommended for use in flow cytometry This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 µl, 1/100, of the suggested working dilution to label 10^6 cells.
- Comment
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BIOTIN(Sigma) Reactivity and.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The conjugate is provided in liquid form in buffer containing Antibody Stabilizer Solution, PBS and 0,09% Sodium azide, pH 7,4.
- Preservative
- Sodium azide
- Precaution of Use
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- Storage
- 4 °C
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- Target
- ITGA4 (Integrin alpha 4 (ITGA4))
- Alternative Name
- CD49d (ITGA4 Products)
- Synonyms
- CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody
- Background
- CD49d (Anti-VLA-alpha-4) recognizes the 150-kDa alpha-chain of very-late antigen (VLA)-4, a member of the integrin family of cell adhesion molecules. VLA-4, like other integrins, is a noncovalently associated heterodimeric glycoprotein composed of alpha and beta subunits and is involved in cell-cell and cell-extracellular matrix interactions. The beta-chain of the VLA-4 complex is the CD29 antigen, a 130-kDa glycoprotein. The CD29 antigen, also known as the beta-1 subunit, is common to the VLA family of integrins. When acting as a matrix receptor, the CD49d antigen binds to CS-1, an alternatively spliced domain of fibronectin. When functioning as a cell receptor, the CD49d antigen binds to the vascular cell- adhesion molecule-1 (VCAM-1). The interaction between the CD49d antigen and VCAM-1 is known to play an important role in stabilizing the adhesion of lymphocytes to endothelial cells and in mediating B- lymphocyte precursor/bone marrow stromal cell adhesion. The CD49d antigen, when associated with the beta integrin, forms a lymphocyte homing receptor for Peyer's patch, binding to the mucosal vascular addressin MAdCAM-1. The CD49d antigen is also involved in CD3-dependent CD4+ T-lymphocyte activation via its interaction with fibronectin.
- Pathways
- Integrin Complex
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