CD11b antibody (APC)

Catalog No. ABIN1112426

Product Details anti-CD11b Antibody (APC)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Please inquire

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Immunogen: C57BL/10 splenocytes

Clone: M1-70

Isotype: IgG2b

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal tless or equal to 0.25 µg per 10^6 cells in 100 µL volume.

Comment:

Allophycocyanin (Abs/ Em: 651/ 662 nm).

Reagent Preparation:

1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add CD11b APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing PBS, 1% BSA and 0.09 % Sodium azide, pH 7.4.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b (ITGAM Products)

Synonyms: CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

Background: CD11b is a 170 kDa glycoprotein, also known as alphaM integrin, Mac-1 alpha subunit, Mol, CR3, or Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (beta2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation