Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
The monoclonal antibody will detect native and denaturated VEGFR-1/Flt-1 in ELISA experiments and on the surface of different Human cell types. The antibody will also detect Mouse Flt-1.
FLT1
Reactivity: Human
IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA (1.5-8 μg/mL IgG1). Western blotting (1-10 μg/mL IgG1). Immunoprecipitation: (1-5 μg/mL IgG1 lysate or reaction volume). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 6.0 without preservatives or stabilizers
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0 °C. Reconstituted anti-VEGFR-1/Flt-1 is stable at 2-8 °C for one month or in working aliquots at -20 °C for longer.
VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor