Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
The antibody will detect human VEGFR-3/FLT-4. In Western blots, this antibody shows a moderate cross-reactivity with VEGFR-2/KDR and a weak cross-reactivity with VEGFR-1/Flt-1.
Purification
Protein A Affinity Chromatography.
Immunogen
Recombinant human soluble FLT-4 protein (110 kDa).
FLT4
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 0.5-1.5 μg/mL, allows the detection of 0.25- 0.5 ng/well rhVEGFR-3/FLT-4. Western Blot: 0.5-1 μg/mL, it will detect approximately 5 ng/lane of rh VEGFR-3/FLT-4 underreducing conditions depending on the visualisation method. Immunoprecipitation: 1-5 μg/mL lysate or reaction volume. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water/PBS to a concentration of > 0.5 mg/mL.
Buffer
PBS, pH 7.4 containing no preservatives or stabilizers.
Preservative
Without preservative
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE.