TNFSF10
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISADirect: To detect hTRAIL/Apo2L by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthTRAIL/Apo2L. Sandwich: To detect hTRAIL/Apo2L by sandwich ELISA (using 100 l/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This BiotinylatedPolyclonal Antibody, in conjunction with Anti-Human TRAIL/Apo2L (PP1084P1 or PP1084P2)as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthTRAIL/Apo2L. Western BlotTo detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Target
TRAIL (TNFSF10)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell's apoptotic machinery.Synonyms: APO2L, Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, TNFSF10, Tumor necrosis factor ligand superfamily member 10