ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr319) (PerCP-Cy5.5)

Details for Product anti-ZAP70 Antibody No. ABIN1176924, Supplier: Log in to see
Antigen
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70
  • tyrosine-protein kinase ZAP-70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T-cell receptor associated protein kinase 70
  • zap70.L
  • zap70
  • ZAP70
  • LOC707555
  • Smp_149470
  • LOC100342021
  • LOC100390922
  • LOC100542877
  • Zap70
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
pTyr319
120
53
50
36
31
26
25
14
12
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7
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1
1
1
1
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Reactivity
Human
683
246
152
14
11
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8
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4
3
1
Host
Mouse
346
319
17
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This ZAP70 antibody is conjugated to PerCP-Cy5.5
34
29
26
23
16
14
9
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Application
Intracellular Staining (ICS)
357
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216
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154
65
52
50
27
8
7
4
3
3
2
2
2
2
Options
Supplier
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Supplier Product No.
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Brand BD Phosflow™
Immunogen Human phosphorylated ZAP70 Peptide
Clone 17A-P
Isotype IgG1
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others ZAP70 products on genomics-online (e.g. as negative or positive controls)
Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background ZAP70 is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. TCR stimulation leads to autophosphorylation of ZAP70 at Tyr-315 amd Tyr-319, and mutation of the Tyr-319 site dramatically impairs TCR signaling. In addition, TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyr-493 in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. Clone 17A/P-ZAP70 recognizes the phosphorylated form of ZAP70 (Y319). It also cross-reacts with SYK (Y352) due to homology of the phosphorylation site with ZAP70 (Y319). The PE-conjugated format has been evaluated using human and mouse model systems. The unconjugated form of the antibody (Cat. No. 612574) has also been shown to work in western blot analysis on human, mouse, and rat cells.
Synonyms: ZAP-70, SRK, STD, TZK, Zeta-chain associated protein kinase, 70kD
Pathways TCR Signaling
Sample Volume 5 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.
Product cited in: Di Bartolo, Mège, Germain, Pelosi, Dufour, Michel, Magistrelli, Isacchi, Acuto: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." in: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, 1999 (PubMed).

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994 (PubMed).

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

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