This antibody recognises a cytoplasmic tyrosine kinase designated csk (c-src tyrosine kinase), which downregulates the activity of the c-src oncoprotein.
Purification
Affinity chromatography
Immunogen
Peptide sequence derived from C-terminus of human CSK. AA Sequence:EQLEHIKTHELHL
Western Blot: 1/200 - 1/500, this antibody detects a band of approximately 60 kDa and 30 kDa in Jurkat cell lysates, Overnight incubation is recommended with this antibody. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
TRIS buffered saline pH 7.3 containing 0.02 % Sodium Azide and 0.5 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Csk is ubiquitously expressed as 2 mRNA species. This antibody detects 2 bands in Western blots of Jurkat cells lysates.Synonyms: C-SRC kinase, Protein-tyrosine kinase CYL, Tyrosine-protein kinase CSK