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PARP1 antibody (Cleavage Site, cleaved)
PARP1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-PARP1 Antibody
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Target
See all PARP1 Antibodies
PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
Binding Specificity
All epitopes for PARP1 antibodies
Cleavage Site, cleaved
Reactivity
All reactivities for PARP1 antibodies
Human
Host
All hosts for PARP1 antibodies
Rabbit
Clonality
All clonalities for PARP1 antibodies
Polyclonal
Conjugate
All conjugates for PARP1 antibodies
This PARP1 antibody is un-conjugated
Application
All applications for PARP1 antibodies
Western Blotting (WB)
Specificity
The polyclonal anti-PARP antibody recognizes the full length and the cleaved fragment of PARP.
Purification
Immunoaffinity
Immunogen
Synthetic peptide corresponding to caspase cleavage site in human PARP.
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Alternatives
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Application Details
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Application Notes
Western Blot: 1-4 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
0.2 mg/mL
Buffer
PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C/-80 °C
Storage Comment
Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
Target Details for PARP1
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Target
PARP1
(Poly (ADP-Ribose) Polymerase 1 (PARP1))
Alternative Name
PARP1 (PARP1 Products )
Synonyms
ADPRT antibody, ADPRT 1 antibody, ADPRT1 antibody, ARTD1 antibody, PARP antibody, PARP-1 antibody, PPOL antibody, pADPRT-1 antibody, Adprt antibody, Parp-1 antibody, 5830444G22Rik antibody, AI893648 antibody, Adprp antibody, Adprt1 antibody, C80510 antibody, parp-1 antibody, sPARP-1 antibody, BEST:LD21673 antibody, CG17685 antibody, CG17696 antibody, CG17718 antibody, CG40411 antibody, D.PARP antibody, Dm.pARTa antibody, Dmel\\CG40411 antibody, LD21673.3prime antibody, PARP1 antibody, dPARP antibody, parp antibody, adprt1 antibody, padprt-1 antibody, ppol antibody, si:dkey-206f10.3 antibody, wu:fc60f12 antibody, zgc:110092 antibody, poly(ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase family, member 1 antibody, Poly-(ADP-ribose) polymerase antibody, poly(ADP-ribose) polymerase 1 L homeolog antibody, PARP1 antibody, Parp1 antibody, Parp antibody, parp1.L antibody, parp1 antibody
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARPs N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1
Gene ID
142
NCBI Accession
NP_001609
UniProt
P09874
Pathways
Apoptosis , Caspase Cascade in Apoptosis , DNA Damage Repair , Production of Molecular Mediator of Immune Response , Maintenance of Protein Location
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