PARP1 antibody (Catalytic Domain, cleaved, N-Term)

Catalog No. ABIN127024

Product Details anti-PARP1 Antibody

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Binding Specificity: Catalytic Domain, cleaved, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.

Purification: Affinity purified

Immunogen: Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunocytochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 (PARP1 Products)

Synonyms: ADPRT antibody, ADPRT 1 antibody, ADPRT1 antibody, ARTD1 antibody, PARP antibody, PARP-1 antibody, PPOL antibody, pADPRT-1 antibody, Adprt antibody, Parp-1 antibody, 5830444G22Rik antibody, AI893648 antibody, Adprp antibody, Adprt1 antibody, C80510 antibody, parp-1 antibody, sPARP-1 antibody, BEST:LD21673 antibody, CG17685 antibody, CG17696 antibody, CG17718 antibody, CG40411 antibody, D.PARP antibody, Dm.pARTa antibody, Dmel\\CG40411 antibody, LD21673.3prime antibody, PARP1 antibody, dPARP antibody, parp antibody, adprt1 antibody, padprt-1 antibody, ppol antibody, si:dkey-206f10.3 antibody, wu:fc60f12 antibody, zgc:110092 antibody, poly(ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase family, member 1 antibody, Poly-(ADP-ribose) polymerase antibody, poly(ADP-ribose) polymerase 1 L homeolog antibody, PARP1 antibody, Parp1 antibody, Parp antibody, parp1.L antibody, parp1 antibody

Background: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavege occurs between ASP214 and Gly 215, which separates PARP's N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1

Gene ID: 142

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location