Lamin A/C antibody

Catalog No. ABIN127068

Product Details anti-Lamin A/C Antibody

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody mainly detects full length (~70 kDa) and in a much lesser extent the cleaved fragments (45 kDa and 23 kDa) of Lamin A/C.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 220 of human Lamin A

Application Details

Application Notes: Immunohistochemistry (15-20 μg/mL). Western blot analysis (0.5-4 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin-A/C (LMNA) (LMNA Products)

Synonyms: CDCD1 antibody, CDDC antibody, CMD1A antibody, CMT2B1 antibody, EMD2 antibody, FPL antibody, FPLD antibody, FPLD2 antibody, HGPS antibody, IDC antibody, LDP1 antibody, LFP antibody, LGMD1B antibody, LMN1 antibody, LMNC antibody, LMNL1 antibody, PRO1 antibody, Dhe antibody, 2459 antibody, 74/76 antibody, CG6944 antibody, D5 antibody, DM[[O]] antibody, Dm antibody, Dm(0) antibody, Dm0 antibody, Dm1 antibody, Dm2 antibody, DmLamin antibody, DmO antibody, Dm[[0]] antibody, Dm[[1]] antibody, Dm[[2]] antibody, Dm[[mit]] antibody, Dm[[o]] antibody, Dmel\\CG6944 antibody, Dmo antibody, LAM antibody, Lam Dm[[0]] antibody, Lam(Dm0) antibody, LamDm[[0]] antibody, LamDm[[o]] antibody, Lam[[Dm0]] antibody, Lamin Dm[[0]] antibody, jf27 antibody, l(2)04643 antibody, l(2)25Ec antibody, l(2)gdh-7 antibody, l(2)gdh7 antibody, l(2)jf27 antibody, lam antibody, lamDm0 antibody, lamDm[[0]] antibody, lamin antibody, lamin Dm0 antibody, lamin Dm[[0]] antibody, misg antibody, nlam antibody, cb948 antibody, fk66d12 antibody, wu:fk66d12 antibody, lmna-A antibody, fpl antibody, idc antibody, lfp antibody, cddc antibody, emd2 antibody, fpld antibody, hgps antibody, ldp1 antibody, lmn1 antibody, lmnc antibody, pro1 antibody, cdcd1 antibody, cmd1a antibody, cmt2b1 antibody, lgmd1b antibody, Lamin-A antibody, lamin-L(III)-like antibody, lamin A/C antibody, lamin A antibody, Lamin antibody, lamin A/C L homeolog antibody, lamin-L(III)-like antibody, LMNA antibody, Lmna antibody, Lam antibody, lmna antibody, lmna.L antibody, LMINA antibody

Background: Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization. Lamin A is specifically cleaved by caspase-6 and therefore serves as a marker for caspase-6 activation. During apoptosis the 70 kDa lamin A is cleaved to a large (40-45 kDa) and small (23 kDa) fragment. The cleavage of lamins results in nuclear disregulation and cell death.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Gene ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus