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RAF1 antibody (pSer259)
RAF1
Reactivity: Human, Mouse, Rat
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-RAF1 Antibody
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Target
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RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
Binding Specificity
All epitopes for RAF1 antibodies
pSer259
Reactivity
All reactivities for RAF1 antibodies
Human, Mouse, Rat
Host
All hosts for RAF1 antibodies
Rabbit
Clonality
All clonalities for RAF1 antibodies
Polyclonal
Conjugate
All conjugates for RAF1 antibodies
This RAF1 antibody is un-conjugated
Application
All applications for RAF1 antibodies
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.
Purification
Affinity purified
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Alternatives
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Application Details
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Application Notes
Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 40 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
0.5 mg/mL
Buffer
PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C/-80 °C
Storage Comment
Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
Target Details for RAF1
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Target
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
Alternative Name
RAF1 (RAF1 Products )
Synonyms
CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody
Background
C-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID
5894
NCBI Accession
NP_002871
UniProt
P04049
Pathways
MAPK Signaling , RTK Signaling , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , cAMP Metabolic Process , Stem Cell Maintenance , Hepatitis C , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling , BCR Signaling
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