Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa, 110 kDa, and the unglycosylated form of 80 kDa.
Purification
Immunoaffinity Chromatography
Immunogen
Synthetic peptide surrounding amino acid 810 of Human TrkB
NTRK2
Reactivity: Human
WB, PLA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 0.5-4 μg/mL. Immunofluorescence: 5-10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Due to the various splice variants, the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa, 110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3 % carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.Synonyms: BDNF/NT-3 growth factors receptor, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKB, TrkB tyrosine kinase