MCM4 antibody (AA 761-863) (Alexa Fluor 555)
-
- Target See all MCM4 Antibodies
- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
-
Binding Specificity
- AA 761-863
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This MCM4 antibody is conjugated to Alexa Fluor 555
-
Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Predicted Reactivity
- Human,Mouse,Rat,Dog,Cow,Pig,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human MCM4
- Isotype
- IgG
- Top Product
- Discover our top product MCM4 Primary Antibody
-
-
- Application Notes
-
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
- Alternative Name
- MCM4/CDC21 (MCM4 Products)
- Synonyms
- cdc21 antibody, CDC21 antibody, CDC54 antibody, NKCD antibody, NKGCD antibody, P1-CDC21 antibody, hCdc21 antibody, 19G antibody, AI325074 antibody, AU045576 antibody, Cdc21 antibody, Mcmd4 antibody, mKIAA4003 antibody, mcdc21 antibody, cb1025 antibody, fc12c09 antibody, fj85g09 antibody, hm:zeh1616 antibody, wu:fc12c09 antibody, wu:fj85g09 antibody, zeh1616 antibody, minichromosome maintenance complex component 4 L homeolog antibody, minichromosome maintenance complex component 4 S homeolog antibody, minichromosome maintenance complex component 4 antibody, mcm4.L antibody, mcm4.S antibody, MCM4 antibody, mcm4 antibody, Mcm4 antibody
- Background
-
Synonyms: CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM 4, DNA replication licensing factor MCM4, hCdc 21, hCdc21, Homolog of S. pombe cell devision cycle 21, KIAA4003, mcdc21, MCM 4, MCM 4 minichromosome maintenance deficient 4, MCM4, MCM4 minichromosome maintenance deficient 4, MCM4 minichromosome maintenance deficient 4, mitotin S. cerevisiae, MCM4_HUMAN, Mcmd4, MGC33310, Minichromosome Maintenance 4, Minichromosome maintenance complex component 4, Minichromosome maintenance deficient S. cerevisiae 4, Minichromosome maintenance deficient 4, Minichromosome maintenance deficient 4 homolog S. cerevisiae, Minichromosome maintenance, S. cerevisiae, homolog of, 4, mKIAA4003, P1 CDC21, P1-CDC21, P1CDC21, 19G antibody AI325074, AU045576, CDC 21, CDC 54.
Background: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis.
- Pathways
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
-