Luciferase antibody (HRP)

Catalog No. ABIN1417480

Product Details anti-Luciferase Antibody (HRP)

Target: Luciferase

Reactivity: Firefly

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Luciferase antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Tag

Cross-Reactivity (Details): Photinus pyralis (Common eastern firefly)

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Firefly Luciferase

Isotype: IgG

Application Details

Application Notes: IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Synonyms: hypothetical protein antibody, luciferase antibody, C25G6.3 antibody, Gbro_2186 antibody

Background:

Synonyms: Luciferin 4-monooxygenase

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Gene ID: 7100